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OCTN2VT, a splice variant of OCTN2, does not transport carnitine because of the retention in the endoplasmic reticulum caused by insertion of 24 amino acids in the first extracellular loop of OCTN2

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Title: OCTN2VT, a splice variant of OCTN2, does not transport carnitine because of the retention in the endoplasmic reticulum caused by insertion of 24 amino acids in the first extracellular loop of OCTN2
Authors: Maekawa, Satoshi Browse this author
Mori, Daisuke Browse this author
Nishiya, Tadashi Browse this author
Takikawa, Osamu Browse this author
Horinouchi, Takahiro Browse this author
Nishimoto, Arata Browse this author →KAKEN DB
Kajita, Emi Browse this author
Miwa, Soichi Browse this author →KAKEN DB
Keywords: OCTN2
OCTN2VT
Splicing
Carnitine
N-glycosylation
Endoplasmic reticulum
Issue Date: Jun-2007
Publisher: Elsevier B.V.
Journal Title: Biochimica et Biophysica Acta (BBA) : Molecular Cell Research
Volume: 1773
Issue: 6
Start Page: 1000
End Page: 1006
Publisher DOI: 10.1016/j.bbamcr.2007.04.005
PMID: 17509700
Abstract: A novel organic cation transporter OCTN2 is indispensable for carnitine transport across plasma membrane and subsequent fatty acid metabolism in the mitochondria. Here, we report a novel splice variant of OCTN2 (OCTN2VT), in which a 72-base-pair sequence located in the first intron of OCTN2 gene was spliced between exons 1 and 2 of OCTN2, causing the insertion of 24 amino acids in the first extracellular loop of OCTN2. Despite the similarity between OCTN2 and OCTN2VT regarding primary structure and tissue distribution, their biochemical characteristics were significantly different. OCTN2 was expressed on the plasma membrane with robust N-glycosylation, whereas OCTN2VT was retained in the endoplasmic reticulum (ER) with poor N-glycosylation. In addition, the retention in the ER caused no carnitine uptake into the cells. These results demonstrate that the biochemical and functional characteristics of OCTN2VT are distinct from OCTN2 due to the insertion of 24 amino acids in the first extracellular loop.
Relation: http://www.sciencedirect.com/science/journal/01674889
Type: article (author version)
URI: http://hdl.handle.net/2115/28237
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 三輪 聡一

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