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OCTN2VT, a splice variant of OCTN2, does not transport carnitine because of the retention in the endoplasmic reticulum caused by insertion of 24 amino acids in the first extracellular loop of OCTN2

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タイトル: OCTN2VT, a splice variant of OCTN2, does not transport carnitine because of the retention in the endoplasmic reticulum caused by insertion of 24 amino acids in the first extracellular loop of OCTN2
著者: Maekawa, Satoshi 著作を一覧する
Mori, Daisuke 著作を一覧する
Nishiya, Tadashi 著作を一覧する
Takikawa, Osamu 著作を一覧する
Horinouchi, Takahiro 著作を一覧する
Nishimoto, Arata 著作を一覧する
Kajita, Emi 著作を一覧する
Miwa, Soichi 著作を一覧する
キーワード: OCTN2
OCTN2VT
Splicing
Carnitine
N-glycosylation
Endoplasmic reticulum
発行日: 2007年 6月
出版者: Elsevier B.V.
誌名: Biochimica et Biophysica Acta (BBA) : Molecular Cell Research
巻: 1773
号: 6
開始ページ: 1000
終了ページ: 1006
出版社 DOI: 10.1016/j.bbamcr.2007.04.005
抄録: A novel organic cation transporter OCTN2 is indispensable for carnitine transport across plasma membrane and subsequent fatty acid metabolism in the mitochondria. Here, we report a novel splice variant of OCTN2 (OCTN2VT), in which a 72-base-pair sequence located in the first intron of OCTN2 gene was spliced between exons 1 and 2 of OCTN2, causing the insertion of 24 amino acids in the first extracellular loop of OCTN2. Despite the similarity between OCTN2 and OCTN2VT regarding primary structure and tissue distribution, their biochemical characteristics were significantly different. OCTN2 was expressed on the plasma membrane with robust N-glycosylation, whereas OCTN2VT was retained in the endoplasmic reticulum (ER) with poor N-glycosylation. In addition, the retention in the ER caused no carnitine uptake into the cells. These results demonstrate that the biochemical and functional characteristics of OCTN2VT are distinct from OCTN2 due to the insertion of 24 amino acids in the first extracellular loop.
Relation (URI): http://www.sciencedirect.com/science/journal/01674889
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/28237
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 三輪 聡一

 

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