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Cloning and sequencing of the cellobiose 2-epimerase gene from an obligatory anaerobe, Ruminococcus albus.

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Title: Cloning and sequencing of the cellobiose 2-epimerase gene from an obligatory anaerobe, Ruminococcus albus.
Authors: Ito, Shigeaki Browse this author
Hamada, Shigeki Browse this author
Yamaguchi, Kozo Browse this author
Umene, Shingo Browse this author
Ito, Hiroyuki Browse this author
Matsui, Hirokazu Browse this author →KAKEN DB
Ozawa, Tadahiro Browse this author
Taguchi, Hidenori Browse this author
Watanabe, Jun Browse this author
Wasaki, Jun Browse this author
Ito, Susumu Browse this author
Keywords: Cellobiose 2-epimerase
Ruminococcus albus
Issue Date: 31-Aug-2007
Publisher: Elsevier Inc.
Journal Title: Biochemical and Biophysical Research Communications
Volume: 360
Issue: 3
Start Page: 640
End Page: 645
Publisher DOI: 10.1016/j.bbrc.2007.06.091
PMID: 17612504
Abstract: Cellobiose 2-epimerase (EC was first identified in 1967 as an extracellular enzyme that catalyzes the reversible epimerization between cellobiose and 4-O-β-d-glucopyranosyl-d-mannose in a culture broth of Ruminococcus albus 7 (ATCC 27210T). Here, for the first time, we describe the purification of cellobiose 2-epimerase from R. albus NE1. The enzyme was found to 2-epimerize the reducing terminal glucose moieties of cellotriose and cellotetraose in addition to cellobiose. The gene encoding cellobiose 2-epimerase comprises 1170 bp (389 amino acids) and is present as a single copy in the genome. The deduced amino acid sequence of the mature enzyme contains the possible catalytic residues Arg52, His243, Glu246, and His374. Sequence analysis shows the gene shares a very low level of homology with N-acetyl-d-glucosamine 2-epimerases (EC, but no significant homology to any other epimerases reported to date.
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 濱田 茂樹

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