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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Cloning and sequencing of the cellobiose 2-epimerase gene from an obligatory anaerobe, Ruminococcus albus.
| Title: | Cloning and sequencing of the cellobiose 2-epimerase gene from an obligatory anaerobe, Ruminococcus albus. |
| Authors: | Ito, Shigeaki Browse this author | | Hamada, Shigeki Browse this author | | Yamaguchi, Kozo Browse this author | | Umene, Shingo Browse this author | | Ito, Hiroyuki Browse this author | | Matsui, Hirokazu Browse this author →KAKEN DB | | Ozawa, Tadahiro Browse this author | | Taguchi, Hidenori Browse this author | | Watanabe, Jun Browse this author | | Wasaki, Jun Browse this author | | Ito, Susumu Browse this author |
| Keywords: | Cellobiose 2-epimerase | | Ruminococcus albus | | Rumen | | Cloning | | Sequencing |
| Issue Date: | 31-Aug-2007 |
| Publisher: | Elsevier Inc. |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 360 |
| Issue: | 3 |
| Start Page: | 640 |
| End Page: | 645 |
| Publisher DOI: | 10.1016/j.bbrc.2007.06.091 |
| PMID: | 17612504 |
| Abstract: | Cellobiose 2-epimerase (EC 5.1.3.11) was first identified in 1967 as an extracellular enzyme that catalyzes the reversible epimerization between cellobiose and 4-O-β-d-glucopyranosyl-d-mannose in a culture broth of Ruminococcus albus 7 (ATCC 27210T). Here, for the first time, we describe the purification of cellobiose 2-epimerase from R. albus NE1. The enzyme was found to 2-epimerize the reducing terminal glucose moieties of cellotriose and cellotetraose in addition to cellobiose. The gene encoding cellobiose 2-epimerase comprises 1170 bp (389 amino acids) and is present as a single copy in the genome. The deduced amino acid sequence of the mature enzyme contains the possible catalytic residues Arg52, His243, Glu246, and His374. Sequence analysis shows the gene shares a very low level of homology with N-acetyl-d-glucosamine 2-epimerases (EC 5.1.3.8), but no significant homology to any other epimerases reported to date. |
| Relation: | http://www.sciencedirect.com/science/journal/0006291X |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/28242 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 濱田 茂樹
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