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Effects of isolation method and pre-treatment with ethylene glycol or raffinose before vitrification on in vitro viability of mouse preantral follicles

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/28268

Title: Effects of isolation method and pre-treatment with ethylene glycol or raffinose before vitrification on in vitro viability of mouse preantral follicles
Authors: Nagano, Masashi Browse this author →KAKEN DB
Atabay, Eufrocina P. Browse this author
Atabay, Edwin C. Browse this author
Hishinuma, Mitsugu Browse this author
Katagiri, Seiji Browse this author →KAKEN DB
Takahashi, Yoshiyuki Browse this author →KAKEN DB
Issue Date: Jun-2007
Publisher: Biomedical Research Press
Journal Title: Biomedical Research
Volume: 28
Issue: 3
Start Page: 153
End Page: 160
Publisher DOI: 10.2220/biomedres.28.153
PMID: 17625348
Abstract: Effects of isolation and vitrification protocols on follicular survival after warming were examined. Mouse preantral follicles enzymatically or mechanically isolated from ovaries of 12-day-old mice were exposed either to 2 M ethylene glycol (EG) for 2 or 5 min, or to ascending concentrations (0.15 then 0.3 M) of raffinose for 2 or 5 min each (2-2 and 5-5 min). They were then exposed to a vitrification solution (VS) composed of 6 M EG and 0.3 M raffinose for 0.5, 1, or 2 min before vitrification. Mechanically isolated follicles showed higher survival than enzymatically isolated follicles, regardless of periods of exposure to EG or raffinose and subsequent exposure to VS. After 10 days of culture, follicular growth and maturational ability of oocytes derived from vitrified follicles exposed to 2 M EG for 5 min and to VS for 1 min were higher than those from follicles exposed to raffinose solutions for 2-2 min and to VS for 1 min. Histological evaluation revealed that exposure of preantral follicles to raffinose solutions caused cytoplasmic vacuolation in granulosa cells which could be due to cellular shrinkage during dehydration; whereas, exposure to 2 M EG induced morphological alterations in follicles only to a lesser extent.
Type: article (author version)
URI: http://hdl.handle.net/2115/28268
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 永野 昌志

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