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Effects of isolation method and pre-treatment with ethylene glycol or raffinose before vitrification on in vitro viability of mouse preantral follicles

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タイトル: Effects of isolation method and pre-treatment with ethylene glycol or raffinose before vitrification on in vitro viability of mouse preantral follicles
著者: Nagano, Masashi 著作を一覧する
Atabay, Eufrocina P. 著作を一覧する
Atabay, Edwin C. 著作を一覧する
Hishinuma, Mitsugu 著作を一覧する
Katagiri, Seiji 著作を一覧する
Takahashi, Yoshiyuki 著作を一覧する
発行日: 2007年 6月
出版者: Biomedical Research Press
誌名: Biomedical Research
巻: 28
号: 3
開始ページ: 153
終了ページ: 160
出版社 DOI: 10.2220/biomedres.28.153
抄録: Effects of isolation and vitrification protocols on follicular survival after warming were examined. Mouse preantral follicles enzymatically or mechanically isolated from ovaries of 12-day-old mice were exposed either to 2 M ethylene glycol (EG) for 2 or 5 min, or to ascending concentrations (0.15 then 0.3 M) of raffinose for 2 or 5 min each (2-2 and 5-5 min). They were then exposed to a vitrification solution (VS) composed of 6 M EG and 0.3 M raffinose for 0.5, 1, or 2 min before vitrification. Mechanically isolated follicles showed higher survival than enzymatically isolated follicles, regardless of periods of exposure to EG or raffinose and subsequent exposure to VS. After 10 days of culture, follicular growth and maturational ability of oocytes derived from vitrified follicles exposed to 2 M EG for 5 min and to VS for 1 min were higher than those from follicles exposed to raffinose solutions for 2-2 min and to VS for 1 min. Histological evaluation revealed that exposure of preantral follicles to raffinose solutions caused cytoplasmic vacuolation in granulosa cells which could be due to cellular shrinkage during dehydration; whereas, exposure to 2 M EG induced morphological alterations in follicles only to a lesser extent.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/28268
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 永野 昌志

 

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