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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
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Detection of altered N-glycan profiles in whole serum from rheumatoid arthritis patients
| Title: | Detection of altered N-glycan profiles in whole serum from rheumatoid arthritis patients |
| Authors: | Nakagawa, Hiroaki Browse this author | | Hato, Megumi Browse this author | | Takegawa, Yasuhiro Browse this author | | Deguchi, Kisaburo Browse this author | | Ito, Hiroki Browse this author | | Takahata, Masahiko Browse this author | | Iwasaki, Norimasa Browse this author →KAKEN DB | | Minami, Akio Browse this author →KAKEN DB | | Nishimura, Shin-Ichiro Browse this author →KAKEN DB |
| Keywords: | N-glycan | | Serum | | Rheumatoid arthritis | | LC–MS |
| Issue Date: | 15-Jun-2007 |
| Publisher: | Elsevier B.V. |
| Journal Title: | Journal of Chromatography B |
| Volume: | 853 |
| Issue: | 1-2 |
| Start Page: | 133 |
| End Page: | 137 |
| Publisher DOI: | 10.1016/j.jchromb.2007.03.003 |
| PMID: | 17392038 |
| Abstract: | Altered N-glycosylation occurs in many diseases. In rheumatoid arthritis (RA), for example, reduction in galactose residues in IgG and an increase in fucose residues in α1-acid glycoprotein have been observed. To further analyse N-glycans in disease, we show N-glycan profiling from whole serum employing reversed phase high performance liquid chromatography/negative-ion mode by sonic spray ionization ion trap mass spectrometry with pyridylamination. Profiles from female 15 RA patients and 18 aged-matched healthy women were compared. The most significant change seen in RA was decreased levels of mono-galactosyl bi-antennary N-glycans, in agreement with the previous reports regarding IgG. We also show previously unreported differences between isomers and increased tri-antennary oligosaccharides. These results indicate that LC–MS analysis of whole serum N-glycans can identify N-glycan alterations in RA and that this is a promising method both for studies of RA mechanisms and diagnosis. |
| Relation: | http://www.sciencedirect.com/science/journal/15700232 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/28276 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 中川 裕章
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