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Detection of altered N-glycan profiles in whole serum from rheumatoid arthritis patients

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タイトル: Detection of altered N-glycan profiles in whole serum from rheumatoid arthritis patients
著者: Nakagawa, Hiroaki 著作を一覧する
Hato, Megumi 著作を一覧する
Takegawa, Yasuhiro 著作を一覧する
Deguchi, Kisaburo 著作を一覧する
Ito, Hiroki 著作を一覧する
Takahata, Masahiko 著作を一覧する
Iwasaki, Norimasa 著作を一覧する
Minami, Akio 著作を一覧する
Nishimura, Shin-Ichiro 著作を一覧する
キーワード: N-glycan
Rheumatoid arthritis
発行日: 2007年 6月15日
出版者: Elsevier B.V.
誌名: Journal of Chromatography B
巻: 853
号: 1-2
開始ページ: 133
終了ページ: 137
出版社 DOI: 10.1016/j.jchromb.2007.03.003
抄録: Altered N-glycosylation occurs in many diseases. In rheumatoid arthritis (RA), for example, reduction in galactose residues in IgG and an increase in fucose residues in α1-acid glycoprotein have been observed. To further analyse N-glycans in disease, we show N-glycan profiling from whole serum employing reversed phase high performance liquid chromatography/negative-ion mode by sonic spray ionization ion trap mass spectrometry with pyridylamination. Profiles from female 15 RA patients and 18 aged-matched healthy women were compared. The most significant change seen in RA was decreased levels of mono-galactosyl bi-antennary N-glycans, in agreement with the previous reports regarding IgG. We also show previously unreported differences between isomers and increased tri-antennary oligosaccharides. These results indicate that LC–MS analysis of whole serum N-glycans can identify N-glycan alterations in RA and that this is a promising method both for studies of RA mechanisms and diagnosis.
Relation (URI):
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 中川 裕章


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