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Detection of altered N-glycan profiles in whole serum from rheumatoid arthritis patients

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Title: Detection of altered N-glycan profiles in whole serum from rheumatoid arthritis patients
Authors: Nakagawa, Hiroaki Browse this author
Hato, Megumi Browse this author
Takegawa, Yasuhiro Browse this author
Deguchi, Kisaburo Browse this author
Ito, Hiroki Browse this author
Takahata, Masahiko Browse this author
Iwasaki, Norimasa Browse this author →KAKEN DB
Minami, Akio Browse this author →KAKEN DB
Nishimura, Shin-Ichiro Browse this author →KAKEN DB
Keywords: N-glycan
Serum
Rheumatoid arthritis
LC–MS
Issue Date: 15-Jun-2007
Publisher: Elsevier B.V.
Journal Title: Journal of Chromatography B
Volume: 853
Issue: 1-2
Start Page: 133
End Page: 137
Publisher DOI: 10.1016/j.jchromb.2007.03.003
PMID: 17392038
Abstract: Altered N-glycosylation occurs in many diseases. In rheumatoid arthritis (RA), for example, reduction in galactose residues in IgG and an increase in fucose residues in α1-acid glycoprotein have been observed. To further analyse N-glycans in disease, we show N-glycan profiling from whole serum employing reversed phase high performance liquid chromatography/negative-ion mode by sonic spray ionization ion trap mass spectrometry with pyridylamination. Profiles from female 15 RA patients and 18 aged-matched healthy women were compared. The most significant change seen in RA was decreased levels of mono-galactosyl bi-antennary N-glycans, in agreement with the previous reports regarding IgG. We also show previously unreported differences between isomers and increased tri-antennary oligosaccharides. These results indicate that LC–MS analysis of whole serum N-glycans can identify N-glycan alterations in RA and that this is a promising method both for studies of RA mechanisms and diagnosis.
Relation: http://www.sciencedirect.com/science/journal/15700232
Type: article (author version)
URI: http://hdl.handle.net/2115/28276
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 中川 裕章

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