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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Dental Medicine / Faculty of Dental Medicine >
Peer-reviewed Journal Articles, etc >
Establishment of cell lines that exhibit pluripotency from miniature swine periodontal ligaments.
| Title: | Establishment of cell lines that exhibit pluripotency from miniature swine periodontal ligaments. |
| Authors: | Ibi, Miho Browse this author | | Ishisaki, Akira Browse this author | | Yamamoto, Matsuo Browse this author | | Wada, Satoshi Browse this author | | Kozakai, Takaharu Browse this author | | Nakashima, Aiko Browse this author | | Iida, Jynichiro Browse this author | | Takao, Sonshin Browse this author | | Izumi, Yuichi Browse this author | | Yokoyama, Atsuro Browse this author | | Tamura, Masato Browse this author →KAKEN DB |
| Keywords: | Periodontal ligament | | Miniature swine | | Endothelial cells | | CD31 | | von Willebrand factor |
| Issue Date: | Oct-2007 |
| Publisher: | Elsevier Ltd. |
| Journal Title: | Archives of Oral Biology |
| Volume: | 52 |
| Issue: | 10 |
| Start Page: | 1002 |
| End Page: | 1008 |
| Publisher DOI: | 10.1016/j.archoralbio.2007.04.009 |
| PMID: | 17543882 |
| Abstract: | Objective: The periodontal ligament (PDL) is a fibrous connective tissue composed of heterogeneous cell types, including PDL fibroblasts. It is not clear whether cells within the PDL fibroblast population retain the potency to differentiate into other cell types. Design: In the present study, clonal cell lines, derived from Clawn miniature swine PDLs, were established by gene transfection for a human telomerase reverse transcriptase, and characterized. Results: These cell lines, denoted TesPDL1–4, had PDL fibroblasts that showed fibroblastic morphology and expressed procollagen α1(I), osteopontin, periostin and alkaline phosphatase mRNA. Under the specific culture conditions, TesPDL3 cells also have the ability to express CD31, vascular endothelial cadherin, von Willebrand factor, osteocalcin, and to form extracellular mineralized nodules. Conclusions: Our data indicate that TesPDL3 cells have unique properties of expressing several phenotype of fibroblasts, vascular endothelial cells and osteoblasts in cultures. |
| Relation: | http://www.sciencedirect.com/science/journal/00039969 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/28279 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 田村 正人
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