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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
Peer-reviewed Journal Articles, etc >
Development of a device to stretch tissue-like materials and to measure their mechanical properties by scanning probe microscopy.
| Title: | Development of a device to stretch tissue-like materials and to measure their mechanical properties by scanning probe microscopy. |
| Authors: | Mizutani, Takeomi Browse this author | | Haga, Hisashi Browse this author →KAKEN DB | | Kawabata, Kazushige Browse this author |
| Keywords: | Scanning probe microscopy | | Collagen gel | | Stiffness | | External loading force | | Epithelial cell |
| Issue Date: | Jul-2007 |
| Publisher: | Elsevier Ltd. |
| Journal Title: | Acta Biomaterialia |
| Volume: | 3 |
| Issue: | 4 |
| Start Page: | 485 |
| End Page: | 493 |
| Publisher DOI: | 10.1016/j.actbio.2006.11.007 |
| PMID: | 17251072 |
| Abstract: | We have developed a new stretch device to investigate the biomechanical responses to an external loading force on a tissue-like material consisting of cells and a collagen gel. Collagen gel, a typical matrix found abundantly in the connective tissue, was attached to an elastic chamber that was precoated with a thin layer of collagen. Madin–Darby canine kidney cells that were cultured on the collagen gel were stretched in a uniaxial direction via deformation of the elastic chamber. Changes in the morphology and stiffness of the tissue-like structure were measured before and after the stretch using wide-range scanning probe microscopy (WR-SPM). The change in cellular morphology was heterogeneous, and there was a twofold increase in the intercellular junction due to the stretch. In addition to the WR-SPM measurements, this device enables observation of the spatial distribution of cytoskeletal proteins such as vimentin and α-catenin using immunofluorescent microscopy. We concluded that the stretch device we have reported in this paper is useful for measuring the mechanical response of a tissue-like material over a range of cell sizes when exposed to an external loading force. |
| Relation: | http://www.sciencedirect.com/science/journal/17427061 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/28420 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 水谷 武臣
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