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Development of a device to stretch tissue-like materials and to measure their mechanical properties by scanning probe microscopy.

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/28420

Title: Development of a device to stretch tissue-like materials and to measure their mechanical properties by scanning probe microscopy.
Authors: Mizutani, Takeomi Browse this author
Haga, Hisashi Browse this author →KAKEN DB
Kawabata, Kazushige Browse this author
Keywords: Scanning probe microscopy
Collagen gel
Stiffness
External loading force
Epithelial cell
Issue Date: Jul-2007
Publisher: Elsevier Ltd.
Journal Title: Acta Biomaterialia
Volume: 3
Issue: 4
Start Page: 485
End Page: 493
Publisher DOI: 10.1016/j.actbio.2006.11.007
PMID: 17251072
Abstract: We have developed a new stretch device to investigate the biomechanical responses to an external loading force on a tissue-like material consisting of cells and a collagen gel. Collagen gel, a typical matrix found abundantly in the connective tissue, was attached to an elastic chamber that was precoated with a thin layer of collagen. Madin–Darby canine kidney cells that were cultured on the collagen gel were stretched in a uniaxial direction via deformation of the elastic chamber. Changes in the morphology and stiffness of the tissue-like structure were measured before and after the stretch using wide-range scanning probe microscopy (WR-SPM). The change in cellular morphology was heterogeneous, and there was a twofold increase in the intercellular junction due to the stretch. In addition to the WR-SPM measurements, this device enables observation of the spatial distribution of cytoskeletal proteins such as vimentin and α-catenin using immunofluorescent microscopy. We concluded that the stretch device we have reported in this paper is useful for measuring the mechanical response of a tissue-like material over a range of cell sizes when exposed to an external loading force.
Relation: http://www.sciencedirect.com/science/journal/17427061
Type: article (author version)
URI: http://hdl.handle.net/2115/28420
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 水谷 武臣

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