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Volume 48, Number 4 >

Relationship among growth, steroid production and immunolocalization of transforming growth factor-β1 in the normally developing mouse follicles cultured in vitro

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.48.4.169

Title: Relationship among growth, steroid production and immunolocalization of transforming growth factor-β1 in the normally developing mouse follicles cultured in vitro
Authors: BISHONGA, Christopher Browse this author
TAKAHASHI, Yoshiyuki Browse this author →KAKEN DB
KATAGIRI, Seiji Browse this author
NAGANO, Masashi Browse this author →KAKEN DB
ISHIKAWA, Akiko Browse this author
Keywords: estradiol
follicle culture
granulosa cells
mouse
Issue Date: 28-Feb-2001
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 48
Issue: 4
Start Page: 169
End Page: 176
Abstract: This study examined the relationship among growth, steroid production and transforming growth factor-β1 (TGF-β1) immunolocalization in the mouse follicles cultured in vitro to evaluate the hypothesis that normally developing follicles should express TGF-β1 in the granulosa cells around the time of antrum formation. Preantral follicles with 151-175μm (large category) and 125-150 μm (small category) of initial diameters were used as models for normal and retarded follicles, respectively. Growth rate and timing of antrum formation in both categories were comparable to those of in-vivo grown follicles. At the time of antrum formation, follicular diameters were similar between the two follicle categories; however, antral follicles from the large category showed larger number of granulosa cells, higher estradiol production and proportion of follicles with TGF-β1 positive granulosa cells. Two days after antrum formation, there were no differences in the number of granulosa cells and the proportions of follicles with TGF-β1 positive granulosa or theca cells between the two categories. Temporal association in large follicles between the increase in estradiol production and proportion of follicles with TGF-β1 positive granulosa cells at the time of antrum formation supports our hypothesis. Furthermore, this study demonstrated the usefulness of the follicle culture system in the investigations of follicular physiology.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2857
Appears in Collections:Japanese Journal of Veterinary Research > Volume 48, Number 4

Submitter: 獣医学部図書室

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