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Volume 49, Number 2 >

Epizootiological survey of hantavirus among rodent species in Ningxia Hui Autonomous Province, China

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.49.2.105

Title: Epizootiological survey of hantavirus among rodent species in Ningxia Hui Autonomous Province, China
Authors: KARIWA, Hiroaki Browse this author
CUI, Bai Zhong Browse this author
ARAKI, Koichi Browse this author
YOSHIMATSU, Kumiko Browse this author
LOKUGAMAGE, Kumari Browse this author
LOKUGAMAGE, Nandadeva Browse this author
MURPHY, Michael E. Browse this author
MIZUTANI, Tetsuya Browse this author
ARIKAWA, Jiro Browse this author →KAKEN DB
FUKUSHIMA, Hiroshi Browse this author
HU, Xiong Browse this author
CHEN, Jiehua Browse this author
TAKASHIMA, Ikuo Browse this author
Keywords: Apodemus agrarius
Hantavirus
Hemorrhagic fever with renal syndrome
Rodent
China
Issue Date: 31-Aug-2001
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 49
Issue: 2
Start Page: 105
End Page: 114
Abstract: Hantaviral antibodies were detected in the sera from Apodemus (A.) agrarius and A. peninsulae captured in Ningxia province, China by several different serological diagnostic methods. A total of 409 sera from rodent and insectivore species were collected in 1999 and examined by indirect immunofluorescent antibody assay (IFA). Among them, 19 of 191 (9.9%) sera of A. agrarius and 1 of 13 (7.7%) sera of A. peninsulae were positive for hantaviral antibodies. The other species (Rattus norvegicus, Mus musculus, Cricetulus triton, and Sorex cylindricauda) were negative. The reaction pattern of positive serum was characterized as scattered and granular virus antigens in the cytoplasm of hantavirus infected Vero E6 cells. Some of the A. agrarius sera positive for hantavirus were further examined by Western blotting (WB), enzyme-linked immunosorbent assay (ELISA), and the focus reduction neutralization test (FRNT). By WB, positive sera showed the same specific reaction pattern of baculovirus-expressed recombinant hantaviral nucleocapsid protein, as shown in hantavirus-immune serum. By ELISA, IFA-positive sera showed significantly higher optical densities (around 1.0) than the negative A. agrarius sera. Hantaan type hantavirus was neutralized with the positive sera. These results suggest that A. agrarius have hantavirus infection and may play a role as a reservoir animal for hantavirus in Ningxia Hui Autonomous Province, China.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2914
Appears in Collections:Japanese Journal of Veterinary Research > Volume 49, Number 2

Submitter: 獣医学部図書室

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