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Volume 49, Number 4 >

Genetic and biological comparision of tick-borne encephalitis viruses from Hokkaido and Far-Eastern Russia

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.49.4.297

Title: Genetic and biological comparision of tick-borne encephalitis viruses from Hokkaido and Far-Eastern Russia
Authors: GOTO, Akiko Browse this author
HAYASAKA, Daisuke Browse this author
YOSHII, Kentarou Browse this author →KAKEN DB
MIZUTANI, Tetsuya Browse this author
KARIWA, Hiroaki Browse this author
TAKASHIMA, Ikuo Browse this author
Keywords: Tick-borne encephalitis virus
flavivirus
genome sequence
biological property
Issue Date: 28-Feb-2002
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 49
Issue: 4
Start Page: 297
End Page: 307
Abstract: We compared the biological properties of Oshima 5-10 (tick-borne encephalitis [TBE] virus isolated in Hokkaido, Japan) and Sofjin-HO (Far-Eastern subtype TBE virus) including plaque formation, virus replication and virus protein synthesis in BHK-21 cell cultures to reveal strain differences. We also determined the complete nucleotide sequences of both strains and compared the deduced amino acid sequences. Plaques of Oshima 5-10 were smaller than those of Sofjin-HO. Virus titers in culture fluid of Oshima 5-10 were 1/100 of those of Sofjin-HO at 9 and 12 hr after infection. Less viral protein and RNA syntheses of strain Oshima 5-10 was observed than with Sofjin-HO. Genetic analysis revealed 1.4% of amino acids to differ with Sofjin-HO. No difference between the two strains was detected in the motif sequence of the viral enzyme, cleavage sites of viral protein or glycosylation sites of NS1.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2927
Appears in Collections:Japanese Journal of Veterinary Research > Volume 49, Number 4

Submitter: 獣医学部図書室

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