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Volume 50, Number 4 >

Effect of activation treatments of recipient oocytes on subsequent development of bovine nuclear transfer embryos

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.50.4.185

Title: Effect of activation treatments of recipient oocytes on subsequent development of bovine nuclear transfer embryos
Authors: Atabay, Edwin C. Browse this author
Katagiri, Seiji Browse this author
Nagano, Masashi Browse this author →KAKEN DB
Takahashi, Yoshiyuki Browse this author →KAKEN DB
Issue Date: 28-Feb-2003
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 50
Issue: 4
Start Page: 185
End Page: 194
Abstract: Effects of recipient oocyte activation methods on the development of nuclear transfer (NT) embryos were investigated. In Exp. 1, cell-cycle phase of serum-starved bovine cumulus cells was examined by flow cytometry. Majority (95.5%) of medium-sized (16-20 μm) cells that made up 56% of total cells was at the G_0/G_1 phase. NT embryos were constructed by electric fusion with the medium-sized serum-starved cumulus cells and bovine oocytes of 3 different preparations: enucleated oocytes treated with calcium ionophore A 23187 for 5 min and cycloheximide for 5 hr (A 23187/CHX), those treated with ethanol for 7 min and cycloheximide for 2 hr (ethanol CHX) and those without treatment. In Exp. 2 and 3, developmental competence of NT embryos constructed with A 23187/CHX- and ethanol/HX-treated oocytes was compared to that of NT embryos constructed with non-treated oocytes, respectively. Further, nuclear behavior in 3 different NT embryos was examined in Exp. 4. Within 1 hr after fusion, majority of the NT embryos constructed with non-treated oocytes showed condensed chromosome. Three hours after fusion, about 50% of NT embryos constructed with non-treated or ethanol/CHX-treated oocytes showed a single pronucleus-like structure. NT embryos constructed with ethanol/CHX-treated oocytes showed similar rates of fusion, cleavage and blastocyst formation to those of the non-treated oocytes. In contrast, NT embryos constructed with A 23187/CHX-treated oocytes did not show any pronucleus-like structure and showed lower cleavage rate and no development to blastocysts. The results indicate that ethanol/CHX-treated oocytes could support development of somatic cell NT embryos to the blastocyst stage at a similar rate to that of non-treated oocytes.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2966
Appears in Collections:Japanese Journal of Veterinary Research > Volume 50, Number 4

Submitter: 獣医学部図書室

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