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Volume 50, Number 4 >

Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for mtDNA typing in Hokkaido brown bear (Ursus aretos yesoensis)

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.50.4.195

Title: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for mtDNA typing in Hokkaido brown bear (Ursus aretos yesoensis)
Authors: Satoh, Yuka Browse this author
Mano, Tsutomu Browse this author
Tsuruga, Hifumi Browse this author
Masuda, Ryuichi Browse this author →KAKEN DB
Matsuhashi, Tamako Browse this author
Onuma, Manabu Browse this author
Suzuki, Masatsugu Browse this author
Ohtaishi, Noriyuki Browse this author
Keywords: Hokkaido brown bear
mitochondrial DNA (mtDNA)
PCR RFLP method
Issue Date: 28-Feb-2003
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 50
Issue: 4
Start Page: 195
End Page: 199
Abstract: To develop an easy method of typing of mitochondrial DNA (mtDNA) in Hokkaido brown bears (Ursus arctos yesoensis), the PCR-RFLP technique was improved using four restriction enzymes: Mbo 1, Cfr 13 I, TspE 1, and Fok 1. This approach identified seven groups of mtDNA haplotypes, HB1/2/5-7, HB3, HB4, HB8/9, HB10/11, HB12 and HB13 from 102 brown bears of northern, central and eastern Hokkaido.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2967
Appears in Collections:Japanese Journal of Veterinary Research > Volume 50, Number 4

Submitter: 獣医学部図書室

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