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Functional Analysis of A Pyoverdine Synthetase from Pseudomonas sp. MIS38

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Title: Functional Analysis of A Pyoverdine Synthetase from Pseudomonas sp. MIS38
Authors: LIM, Siew Ping Browse this author
ROONGSAWANG, Niran Browse this author
WASHIO, Kenji Browse this author →KAKEN DB
MORIKAWA, Masaaki Browse this author →KAKEN DB
Keywords: fluorescent Pseudomonas
pyoverdine synthetase
nonribosomal peptide
gene disruption
arthrofactin
Issue Date: Aug-2007
Publisher: Japan Society for Bioscience, Biotechnology, and Agrochemistry
Journal Title: Bioscience, Biotechnology, and Biochemistry
Volume: 71
Issue: 8
Start Page: 2002
End Page: 2009
Publisher DOI: 10.1271/bbb.70185
PMID: 17690457
Abstract: Fluorescent Pseudomonas sp. MIS38 produces a cyclic lipopeptide, arthrofactin. Arthrofactin is synthesized by a unique nonribosomal peptide synthetase (NRPS) with dual C/E-domains. In this study, another class of cyclic peptide, pyoverdine, was isolated from MIS38, viz., Pvd38. The main fraction of Pvd38 had an m⁄z value of 1,064.57 and contained Ala, Glu, Gly, (OHOrn), Ser, and Thr at a ratio of 2:1:1:(1):1:1 in the peptide part, suggesting a new structure compound. A gene encoding NRPS for the chromophore part of Pvd38 was identified, and we found that it contained a conventional E-domain. Gene disruption completely impaired the production of Pvd38, demonstrating that the synthetase is functional. This observation allows us to conclude that different NRPS systems with dual C/E-domains (in arthrofactin synthetase) and a conventional E-domain (in pyoverdine synthetase) are both functional in MIS38.
Relation: http://www.jstage.jst.go.jp/
Type: article
URI: http://hdl.handle.net/2115/29888
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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