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Gentisate 1,2-dioxygenase from Xanthobacter polyaromaticivorans 127W.

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Title: Gentisate 1,2-dioxygenase from Xanthobacter polyaromaticivorans 127W.
Authors: Hirano, Shin-ichi Browse this author
Morikawa, Masaaki Browse this author →KAKEN DB
Takano, Kazufumi Browse this author
Imanaka, Tadayuki Browse this author
Kanaya, Shigenori Browse this author
Keywords: gentisate 1,2-dioxygenase
ferric ion
Issue Date: Jan-2007
Publisher: Japan Society for Bioscience, Biotechnology, and Agrochemistry
Journal Title: Bioscience, Biotechnology, and Biochemistry
Volume: 71
Issue: 1
Start Page: 192
End Page: 199
Publisher DOI: 10.1271/bbb.60444
PMID: 17213679
Abstract: A putative gentisate 1,2-dioxygenase was encoded in the dibenzothiophene degradation gene cluster (dbd) from Xanthobacter polyaromaticivorans 127W. The deduced amino acid sequence showed high sequence similarity with gentisate dioxygenases from Pseudomonas alcaligenes (AAD49427, 65% identical), Bradyrhizobium japonicum (NP_766750, 64%), and P. aeruginosa (ZP_00135722, 54%), and moderate similarity with 1-hydroxy-2-naphthoate dioxygenase from Nocardioides sp. KP7 (BAA31235, 33%) and salicylate dioxygenase from Pseudaminobacter salicylatoxidans (AAQ91293, 33%). The enzyme, GDOxp, was heterologously produced in Escherichia coli and purified to homogeneity. GDOxp formed a tetramer and exhibited high dioxygenase activity against 1,4-dihydroxy 2-naphthoate as well as gentisate, suggesting unusually broad substrate specificity. GDOxp easily released ferrous ion under unfavorable temperature and pH conditions to become an inactive monomer protein. An inactive monomer protein can reconstitute a tetramer structure and restore enzyme activity in a cooperative manner upon the addition of ferrous ion. Chymotryptic digestion and protein truncation experiments suggested that the N-terminal region is important for the tetramerization of GDOxp.
Type: article
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 森川 正章

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