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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Molecular Cloning of cDNAs and Genes for Three α-Glucosidases from European Honeybees, Apis mellifera L., and Heterologous Production of Recombinant Enzymes in Pichia pastoris
| Title: | Molecular Cloning of cDNAs and Genes for Three α-Glucosidases from European Honeybees, Apis mellifera L., and Heterologous Production of Recombinant Enzymes in Pichia pastoris |
| Authors: | NISHIMOTO, Mamoru Browse this author | | MORI, Haruhide Browse this author | | MOTEKI, Tsuneharu Browse this author | | TAKAMURA, Yukiko Browse this author | | IWAI, Gaku Browse this author | | MIYAGUCHI, Yu Browse this author | | OKUYAMA, Masayuki Browse this author →KAKEN DB | | WONGCHAWALIT, Jintanart Browse this author | | SURARIT, Rudee Browse this author | | SVASTI, Jisnuson Browse this author | | KIMURA, Atsuo Browse this author | | CHIBA, Seiya Browse this author |
| Keywords: | honeybee α-glucosidase | | Apis mellifera | | cloning of α-glucosidase | | Pichia pastoris |
| Issue Date: | Jul-2007 |
| Publisher: | Japan Society for Bioscience, Biotechnology, and Agrochemistry |
| Journal Title: | Bioscience, Biotechnology, and Biochemistry |
| Volume: | 71 |
| Issue: | 7 |
| Start Page: | 1703 |
| End Page: | 1716 |
| Publisher DOI: | 10.1271/bbb.70125 |
| PMID: | 17617712 |
| Abstract: | cDNAs encoding three α-glucosidases (HBGases I, II, and III) from European honeybees, Apis mellifera, were cloned and sequenced, two of which were expressed in Pichia pastoris. The cDNAs for HBGases I, II, and III were 1,986, 1,910, and 1,915 bp in length, and included ORFs of 1,767, 1,743, and 1,704 bp encoding polypeptides comprised of 588, 580, and 567 amino acid residues, respectively. The deduced proteins of HBGases I, II, and III contained 18, 14, and 8 putative N-linked glycosylation sites, respectively, but at least 2 sites in HBGase II were unmodified by N-linked oligosaccharide. In spite of remarkable differences in the substrate specificities of the three HBGases, high homologies (38–44% identity) were found in the deduced amino acid sequences. In addition, three genomic DNAs, of 13,325, 2,759, and 27,643 bp, encoding HBGases I, II, and III, respectively, were isolated from honeybees, and the sequences were analyzed. The gene of HBGase I was found to be composed of 8 exons and 7 introns. The gene of HBGase II was not divided by intron. The gene of HBGase III was confirmed to be made up of 9 exons and 8 introns, and to be located in the region upstream the gene of HBGase I. |
| Relation: | http://www.jstage.jst.go.jp/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/30119 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 木村 淳夫
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