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Alanine production in an H+-ATPase- and lactate dehydrogenase-defective mutant of Escherichia coli expressing alanine dehydrogenase

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Title: Alanine production in an H+-ATPase- and lactate dehydrogenase-defective mutant of Escherichia coli expressing alanine dehydrogenase
Authors: Wada, Masaru Browse this author →KAKEN DB
Narita, Kotomi Browse this author
Yokota, Atsushi Browse this author →KAKEN DB
Keywords: Alanine production
Escherichia coli
H+-ATPase
Alanine dehydrogenase
Issue Date: Sep-2007
Publisher: Springer Berlin / Heidelberg
Journal Title: Applied Microbiology and Biotechnology
Volume: 76
Issue: 4
Start Page: 819
End Page: 825
Publisher DOI: 10.1007/s00253-007-1065-y
PMID: 17583806
Abstract: Previously, we reported that pyruvate production was markedly improved in TBLA-1, an H+-ATPase-defective Escherichia coli mutant derived from W1485lip2, a pyruvate-producing E. coli K-12 strain. TBLA-1 produced more than 30 g/l pyruvate from 50 g/l glucose by jar fermentation, while W1485lip2 produced only 25 g/l pyruvate (Yokota et al. in Biosci Biotechnol Biochem 58:2164–2167, 1994b). In this study, we tested the ability of TBLA-1 to produce alanine by fermentation. The alanine dehydrogenase (ADH) gene from Bacillus stearothermophilus was introduced into TBLA-1, and direct fermentation of alanine from glucose was carried out. However, a considerable amount of lactate was also produced. To reduce lactate accumulation, we knocked out the lactate dehydrogenase gene (ldhA) in TBLA-1. This alanine dehydrogenase-expressing and lactate dehydrogenase-defective mutant of TBLA-1 produced 20 g/l alanine from 50 g/l glucose after 24 h of fermentation. The molar conversion ratio of glucose to alanine was 41%, which is the highest level of alanine production reported to date. This is the first report to show that an H+-ATPase-defective mutant of E. coli can be used for amino acid production. Our results further indicate that H+-ATPase-defective mutants may be used for fermentative production of various compounds, including alanine.
Rights: The original publication is available at www.springerlink.com
Type: article (author version)
URI: http://hdl.handle.net/2115/30121
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 和田 大

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