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Crystallization and preliminary X-ray analysis of Streptococcus mutans dextran glucosidase

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Title: Crystallization and preliminary X-ray analysis of Streptococcus mutans dextran glucosidase
Authors: Saburi, Wataru Browse this author →KAKEN DB
Hondoh, Hironori Browse this author
Unno, Hideaki Browse this author
Okuyama, Masayuki Browse this author →KAKEN DB
Mori, Haruhide Browse this author →KAKEN DB
Nakada, Toshitaka Browse this author
Matsuura, Yoshiki Browse this author
Kimura, Atsuo Browse this author →KAKEN DB
Keywords: dextran glucosidase
Streptococcus mutans
α-amylase family
Issue Date: Sep-2007
Publisher: International Union of Crystallography
Journal Title: Acta Crystallographica Section F : Structural Biology and Crystallization Communications
Volume: 63
Issue: 9
Start Page: 774
End Page: 776
Publisher DOI: 10.1107/S174430910703936X
PMID: 17768352
Abstract: Dextran glucosidase from Streptococcus mutans is an exo-hydrolase that acts on the nonreducing terminal α-1,6-glucosidic linkage of oligosaccharides and dextran with a high degree of transglucosylation. Based on amino-acid sequence similarity, this enzyme is classified into glycoside hydrolase family 13. Recombinant dextran glucosidase was purified and crystallized by the hanging-drop vapour-diffusion technique using polyethylene glycol 6000 as a precipitant. The crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 72.72, b = 86.47, c = 104.30 Å. A native data set was collected to 2.2 Å resolution from a single crystal.
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 本同 宏成

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