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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Crystallization and preliminary X-ray analysis of Streptococcus mutans dextran glucosidase
| Title: | Crystallization and preliminary X-ray analysis of Streptococcus mutans dextran glucosidase |
| Authors: | Saburi, Wataru Browse this author →KAKEN DB | | Hondoh, Hironori Browse this author | | Unno, Hideaki Browse this author | | Okuyama, Masayuki Browse this author →KAKEN DB | | Mori, Haruhide Browse this author →KAKEN DB | | Nakada, Toshitaka Browse this author | | Matsuura, Yoshiki Browse this author | | Kimura, Atsuo Browse this author →KAKEN DB |
| Keywords: | dextran glucosidase | | Streptococcus mutans | | α-amylase family |
| Issue Date: | Sep-2007 |
| Publisher: | International Union of Crystallography |
| Journal Title: | Acta Crystallographica Section F : Structural Biology and Crystallization Communications |
| Volume: | 63 |
| Issue: | 9 |
| Start Page: | 774 |
| End Page: | 776 |
| Publisher DOI: | 10.1107/S174430910703936X |
| PMID: | 17768352 |
| Abstract: | Dextran glucosidase from Streptococcus mutans is an exo-hydrolase that acts on the nonreducing terminal α-1,6-glucosidic linkage of oligosaccharides and dextran with a high degree of transglucosylation. Based on amino-acid sequence similarity, this enzyme is classified into glycoside hydrolase family 13. Recombinant dextran glucosidase was purified and crystallized by the hanging-drop vapour-diffusion technique using polyethylene glycol 6000 as a precipitant. The crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 72.72, b = 86.47, c = 104.30 Å. A native data set was collected to 2.2 Å resolution from a single crystal. |
| Relation: | http://journals.iucr.org/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/30136 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 本同 宏成
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