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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
Peer-reviewed Journal Articles, etc >
Gelatinase A and membrane-type matrix metalloproteinases 1 and 2 are responsible for follicle rupture during ovulation in the medaka
| Title: | Gelatinase A and membrane-type matrix metalloproteinases 1 and 2 are responsible for follicle rupture during ovulation in the medaka |
| Authors: | Ogiwara, Katsueki Browse this author | | Takano, Naoharu Browse this author | | Shinohara, Masakazu Browse this author | | Murakami, Masahiro Browse this author | | Takahashi, Takayuki Browse this author →KAKEN DB |
| Keywords: | ovulation | | follicle rupture | | matrix metalloproteinases | | medaka fish |
| Issue Date: | 14-Jun-2005 |
| Publisher: | The National Academy of Sciences of the United States of America |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 102 |
| Issue: | 24 |
| Start Page: | 8442 |
| End Page: | 8447 |
| Publisher DOI: | 10.1073/pnas.0502423102 |
| PMID: | 15941829 |
| Abstract: | Identification of the hydrolytic enzymes involved in follicle rupture during vertebrate ovulation remains a central challenge for research in reproductive biology. Here, we report a previously uncharacterized approach to this problem by using an in vitro ovulation system in the medaka, Oryzias latipes, which is a small freshwater teleost. We found that follicle rupture in the medaka ovary involves the cooperation of at least three matrix metalloproteinases (MMPs), together with the tissue inhibitor of metalloproteinase-2b protein. We determined the discrete roles of each of these proteins during follicle rupture. Our results indicated that gelatinase A induces the hydrolysis of type IV collagen constituting the basement membrane, membrane-type 2 MMP degrades type I collagen present in the theca cell layer, and MT1-MMP and the tissue inhibitor of metalloproteinase-2b are involved in the production and regulation of gelatinase A. These findings will help clarify the mechanism of follicle wall degradation during ovulation in mammalian species. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/30154 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 荻原 克益
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