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Gelatinase A and membrane-type matrix metalloproteinases 1 and 2 are responsible for follicle rupture during ovulation in the medaka

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タイトル: Gelatinase A and membrane-type matrix metalloproteinases 1 and 2 are responsible for follicle rupture during ovulation in the medaka
著者: Ogiwara, Katsueki 著作を一覧する
Takano, Naoharu 著作を一覧する
Shinohara, Masakazu 著作を一覧する
Murakami, Masahiro 著作を一覧する
Takahashi, Takayuki 著作を一覧する
キーワード: ovulation
follicle rupture
matrix metalloproteinases
medaka fish
発行日: 2005年 6月14日
出版者: The National Academy of Sciences of the United States of America
誌名: Proceedings of the National Academy of Sciences of the United States of America
巻: 102
号: 24
開始ページ: 8442
終了ページ: 8447
出版社 DOI: 10.1073/pnas.0502423102
抄録: Identification of the hydrolytic enzymes involved in follicle rupture during vertebrate ovulation remains a central challenge for research in reproductive biology. Here, we report a previously uncharacterized approach to this problem by using an in vitro ovulation system in the medaka, Oryzias latipes, which is a small freshwater teleost. We found that follicle rupture in the medaka ovary involves the cooperation of at least three matrix metalloproteinases (MMPs), together with the tissue inhibitor of metalloproteinase-2b protein. We determined the discrete roles of each of these proteins during follicle rupture. Our results indicated that gelatinase A induces the hydrolysis of type IV collagen constituting the basement membrane, membrane-type 2 MMP degrades type I collagen present in the theca cell layer, and MT1-MMP and the tissue inhibitor of metalloproteinase-2b are involved in the production and regulation of gelatinase A. These findings will help clarify the mechanism of follicle wall degradation during ovulation in mammalian species.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/30154
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 高橋 孝行

 

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