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Gelatinase A and membrane-type matrix metalloproteinases 1 and 2 are responsible for follicle rupture during ovulation in the medaka

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/30154

Title: Gelatinase A and membrane-type matrix metalloproteinases 1 and 2 are responsible for follicle rupture during ovulation in the medaka
Authors: Ogiwara, Katsueki Browse this author
Takano, Naoharu Browse this author
Shinohara, Masakazu Browse this author
Murakami, Masahiro Browse this author
Takahashi, Takayuki Browse this author →KAKEN DB
Keywords: ovulation
follicle rupture
matrix metalloproteinases
medaka fish
Issue Date: 14-Jun-2005
Publisher: The National Academy of Sciences of the United States of America
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 102
Issue: 24
Start Page: 8442
End Page: 8447
Publisher DOI: 10.1073/pnas.0502423102
PMID: 15941829
Abstract: Identification of the hydrolytic enzymes involved in follicle rupture during vertebrate ovulation remains a central challenge for research in reproductive biology. Here, we report a previously uncharacterized approach to this problem by using an in vitro ovulation system in the medaka, Oryzias latipes, which is a small freshwater teleost. We found that follicle rupture in the medaka ovary involves the cooperation of at least three matrix metalloproteinases (MMPs), together with the tissue inhibitor of metalloproteinase-2b protein. We determined the discrete roles of each of these proteins during follicle rupture. Our results indicated that gelatinase A induces the hydrolysis of type IV collagen constituting the basement membrane, membrane-type 2 MMP degrades type I collagen present in the theca cell layer, and MT1-MMP and the tissue inhibitor of metalloproteinase-2b are involved in the production and regulation of gelatinase A. These findings will help clarify the mechanism of follicle wall degradation during ovulation in mammalian species.
Type: article (author version)
URI: http://hdl.handle.net/2115/30154
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高橋 孝行

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