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Antibody neutralization of TGF-β enhances the deterioration of collagen fascicles in a tissue-cultured tendon matrix with ex vivo fibroblast infiltration

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Title: Antibody neutralization of TGF-β enhances the deterioration of collagen fascicles in a tissue-cultured tendon matrix with ex vivo fibroblast infiltration
Authors: Azuma, Chinatsu Browse this author
Tohyama, Harukazu Browse this author →KAKEN DB
Nakamura, Hideki Browse this author
Kanaya, Fuminori Browse this author
Yasuda, Kazunori Browse this author →KAKEN DB
Keywords: Collagen
Fibroblast
Patellar tender
TGF-β
Tissue engineering
Issue Date: 2007
Publisher: Elsevier Ltd.
Journal Title: Journal of Biomechanics
Volume: 40
Issue: 10
Start Page: 2184
End Page: 2190
Publisher DOI: 10.1016/j.jbiomech.2006.10.023
PMID: 17462658
Abstract: A tissue-cultured tendon matrix infiltrated with cultured fibroblasts can be regarded as an ideal tissue-engineered tendon model. To clarify the role of TGF-β in a tissue-cultured tendon matrix during ex vivo cellular infiltration, the present ex vivo study was conducted to test the following hypothesis that antibody neutralization of TGF-β enhances weakening of the collagen fascicles of the patellar tendon matrix in response to ex vivo fibroblast infiltration. In skeletally mature female rabbits, fibroblasts were isolated from the right patellar tendons using an explant culture technique, and the left patellar tendons underwent multiple freeze/thaw treatment with liquid nitrogen to obtain an acellular tendon matrix. Each acellular tendon was placed in a collagen gel containing cultured fibroblasts and then incubated with or without anti-TGF-β1 antibody for 6 weeks. We found that antibody neutralization of TGF-β enhanced the decrease in the tensile strength and tensile modulus of the collagen fascicles of the patellar tendon matrix in response to ex vivo fibroblast infiltration. The present study indicates a possibility that TGF-β may have a role in suppressing the material deterioration of the fascicles in the tendon during ex vivo cellular infiltration.
Relation: http://www.sciencedirect.com/science/journal/00219290
Type: article (author version)
URI: http://hdl.handle.net/2115/30163
Appears in Collections:北海道大学病院 (Hokkaido University Hospital) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 遠山 晴一

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