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Antibody neutralization of TGF-β enhances the deterioration of collagen fascicles in a tissue-cultured tendon matrix with ex vivo fibroblast infiltration

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タイトル: Antibody neutralization of TGF-β enhances the deterioration of collagen fascicles in a tissue-cultured tendon matrix with ex vivo fibroblast infiltration
著者: Azuma, Chinatsu 著作を一覧する
Tohyama, Harukazu 著作を一覧する
Nakamura, Hideki 著作を一覧する
Kanaya, Fuminori 著作を一覧する
Yasuda, Kazunori 著作を一覧する
キーワード: Collagen
Fibroblast
Patellar tender
TGF-β
Tissue engineering
発行日: 2007年
出版者: Elsevier Ltd.
誌名: Journal of Biomechanics
巻: 40
号: 10
開始ページ: 2184
終了ページ: 2190
出版社 DOI: 10.1016/j.jbiomech.2006.10.023
抄録: A tissue-cultured tendon matrix infiltrated with cultured fibroblasts can be regarded as an ideal tissue-engineered tendon model. To clarify the role of TGF-β in a tissue-cultured tendon matrix during ex vivo cellular infiltration, the present ex vivo study was conducted to test the following hypothesis that antibody neutralization of TGF-β enhances weakening of the collagen fascicles of the patellar tendon matrix in response to ex vivo fibroblast infiltration. In skeletally mature female rabbits, fibroblasts were isolated from the right patellar tendons using an explant culture technique, and the left patellar tendons underwent multiple freeze/thaw treatment with liquid nitrogen to obtain an acellular tendon matrix. Each acellular tendon was placed in a collagen gel containing cultured fibroblasts and then incubated with or without anti-TGF-β1 antibody for 6 weeks. We found that antibody neutralization of TGF-β enhanced the decrease in the tensile strength and tensile modulus of the collagen fascicles of the patellar tendon matrix in response to ex vivo fibroblast infiltration. The present study indicates a possibility that TGF-β may have a role in suppressing the material deterioration of the fascicles in the tendon during ex vivo cellular infiltration.
関連URI: http://www.sciencedirect.com/science/journal/00219290
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/30163
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 遠山 晴一

 

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