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Development of an Immunochromatographic Kit for Rapid Diagnosis of H5 Avian Influenza Virus Infection

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タイトル: Development of an Immunochromatographic Kit for Rapid Diagnosis of H5 Avian Influenza Virus Infection
著者: Tsuda, Yoshimi 著作を一覧する
Sakoda, Yoshihiro 著作を一覧する
Sakabe, Saori 著作を一覧する
Mochizuki, Tsuyoshi 著作を一覧する
Namba, Yasuharu 著作を一覧する
Kida, Hiroshi 著作を一覧する
キーワード: Influenza A virus
H5 HA antigen
Diagnosis kit
発行日: 2007年
出版者: Center for Academic Publications Japan
巻: 51
号: 9
開始ページ: 903
終了ページ: 907
抄録: Highly pathogenic avian influenza (HPAI) caused by the H5N1 subtype has given rise to serious damage in poultry industries in Asia. The virus has expanded its geographical range to Europe and Africa, posing a great risk to human health as well. For the control of avian influenza, a rapid diagnosis by detecting the causative virus and identifying its subtype is essential. In the present study, a rapid diagnosis kit combining immunochromatography with enzyme immunoassay which detects the H5 HA antigen of influenza A virus was developed using newly established anti-H5 HA monoclonal antibodies. The present kit specifically detected all of the H5 influenza viruses tested, and did not react with the other HA subtypes. H5 HA antigens were detected from swabs and tissue homogenates of chickens infected with HPAI virus strain A/chicken/Yamaguchi/7/04 (H5N1) from 2 days post inoculation. The kit showed enough sensitivity and specificity for the rapid diagnosis of HPAI.
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 津田 祥美


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