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Signaling adaptor protein Crk is indispensable for malignant feature of glioblastoma cell line KMG4

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タイトル: Signaling adaptor protein Crk is indispensable for malignant feature of glioblastoma cell line KMG4
著者: Wang, Lei 著作を一覧する
Tabu, Kouichi 著作を一覧する
Kimura, Taichi 著作を一覧する
Tsuda, Masumi 著作を一覧する
Linghu, Hua 著作を一覧する
Tanino, Mishie 著作を一覧する
Kaneko, Sadao 著作を一覧する
Nishihara, Hiroshi 著作を一覧する
Tanaka, Shinya 著作を一覧する
キーワード: Crk
発行日: 2007年11月 3日
出版者: Elsevier Inc.
誌名: Biochemical and Biophysical Research Communications
巻: 362
号: 4
開始ページ: 976
終了ページ: 981
出版社 DOI: 10.1016/j.bbrc.2007.08.106
抄録: Signaling adaptor protein Crk has been shown to be involved in pathogenesis of human cancers including brain tumor where Crk was reported to be overexpressed. In this study, we addressed whether Crk is indispensable for malignant phenotype of brain tumor. In 20 surgical specimens of glioma, mRNA of both CrkI and CrkII was found to be elevated in malignant tumor. To define a precise role of Crk, we have established Crk-knockdown cell lines of glioblastoma KMG4 by siRNA, and early phase of cell adhesion to laminin was found to be suppressed. Wound healing assay revealed the decreased cell motility in Crk knockdown cells, and suppression of both anchorage-dependent and -independent growth were demonstrated in these cells. Furthermore, in vivo tumor forming potential was also markedly suppressed. These results suggest that Crk is required for early attachment to laminin, cell motility, and growth of glioblastoma cell line KMG4.
Relation (URI):
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 田中 伸哉


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