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Signaling adaptor protein Crk is indispensable for malignant feature of glioblastoma cell line KMG4

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Title: Signaling adaptor protein Crk is indispensable for malignant feature of glioblastoma cell line KMG4
Authors: Wang, Lei Browse this author
Tabu, Kouichi Browse this author
Kimura, Taichi Browse this author
Tsuda, Masumi Browse this author
Linghu, Hua Browse this author
Tanino, Mishie Browse this author
Kaneko, Sadao Browse this author
Nishihara, Hiroshi Browse this author
Tanaka, Shinya Browse this author →KAKEN DB
Keywords: Crk
Issue Date: 3-Nov-2007
Publisher: Elsevier Inc.
Journal Title: Biochemical and Biophysical Research Communications
Volume: 362
Issue: 4
Start Page: 976
End Page: 981
Publisher DOI: 10.1016/j.bbrc.2007.08.106
PMID: 17825249
Abstract: Signaling adaptor protein Crk has been shown to be involved in pathogenesis of human cancers including brain tumor where Crk was reported to be overexpressed. In this study, we addressed whether Crk is indispensable for malignant phenotype of brain tumor. In 20 surgical specimens of glioma, mRNA of both CrkI and CrkII was found to be elevated in malignant tumor. To define a precise role of Crk, we have established Crk-knockdown cell lines of glioblastoma KMG4 by siRNA, and early phase of cell adhesion to laminin was found to be suppressed. Wound healing assay revealed the decreased cell motility in Crk knockdown cells, and suppression of both anchorage-dependent and -independent growth were demonstrated in these cells. Furthermore, in vivo tumor forming potential was also markedly suppressed. These results suggest that Crk is required for early attachment to laminin, cell motility, and growth of glioblastoma cell line KMG4.
Type: article (author version)
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 田中 伸哉

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