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Isolation and characterization of bacteria from soil contaminated with diesel oil and the possible use of these in autochthonous bioaugmentation

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タイトル: Isolation and characterization of bacteria from soil contaminated with diesel oil and the possible use of these in autochthonous bioaugmentation
著者: Ueno, Akio 著作を一覧する
Ito, Yukiya 著作を一覧する
Yumoto, Isao 著作を一覧する
Okuyama, Hidetoshi 著作を一覧する
キーワード: Autochthonous bioaugmentation
Biostimulation
Diesel oil degradation
Soil intrinsic bacteria
Soil microcosms
発行日: 2007年12月
出版者: Springer Netherlands
誌名: World Journal of Microbiology and Biotechnology
巻: 23
号: 12
開始ページ: 1739
終了ページ: 1745
出版社 DOI: 10.1007/s11274-007-9423-6
抄録: Two bacterial species (isolates N and O) were isolated from a paddy soil microcosm that had been artificially contaminated with diesel oil to which extrinsic Pseudomonas aeruginosa strain WatG, had been added exogenously. One bacterial species (isolate J) was isolated from a similar soil microcosm that had been biostimulated with Luria–Bertani (LB) medium. Isolates N and O, which were tentatively identified as Stenotrophomonas sp. and Ochromonas sp., respectively, by sequencing of their 16 S rRNA genes had no ability to degrade diesel oil on their own in any liquid medium. When each strain was cocultivated with P. aeruginosa strain WatG in liquid mineral salts medium (MSM) containing 1% diesel oil, isolate N enhanced the degradation of diesel oil by P. aeruginosa strain WatG, but isolate O inhibited it. In contrast, isolate J, which was tentatively identified as a Rhodococcus sp., degraded diesel oil contained not only in liquid LB and MSM, but also in paddy soil microcosms supplemented with LB medium. The bioaugmentation capacity of isolate J in soil microcosms contaminated with diesel oil was much higher than that of P. aeruginosa strain WatG. The possibility of using isolate J for autochthonous bioaugmentation is discussed.
Rights: The original publication is available at www.springerlink.com
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/30188
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 奥山 英登志

 

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