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Chromosome elimination in the interspecific hybrid medaka between Oryzias latipes and O. hubbsi

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Title: Chromosome elimination in the interspecific hybrid medaka between Oryzias latipes and O. hubbsi
Authors: Sakai, C. Browse this author
Konno, F. Browse this author
Nakano, O. Browse this author
Iwai, T. Browse this author
Yokota, T. Browse this author
Lee, J. Browse this author
Nishida-Umehara, C. Browse this author →KAKEN DB
Kuroiwa, A. Browse this author
Matsuda, Y. Browse this author
Yamashita, M. Browse this author
Keywords: fluorescent in situ hybridization
hybrid cell
lagging chromosome
phospho-histone H3
rice fish
Issue Date: Oct-2007
Publisher: Springer Netherlands
Journal Title: Chromosome Research
Volume: 15
Issue: 6
Start Page: 697
End Page: 709
Publisher DOI: 10.1007/s10577-007-1155-9
PMID: 17603754
Abstract: An interspecific hybrid medaka (rice fish) between Oryzias latipes and O. hubbsi is embryonically lethal. To gain an insight into the cellular and molecular mechanisms that cause the abnormalities occurring in the hybrid medaka, we investigated the behavior of chromosomes and the expression patterns of proteins responsible for the chromosome behavior. The number of chromosomes in the hybrid embryos gradually decreased to nearly half, since abnormal cell division with lagging chromosomes at anaphase eliminated the chromosomes from the cells. The chromosome lagging occurred at the first cleavage and continued throughout embryogenesis even after the midblastula transition. Fluorescent in-situ hybridization analyses revealed that the chromosomes derived from O. hubbsi are preferentially eliminated in both O. latipes–hubbsi and O. hubbsi–latipes embryos. Whole-mount immunocytochemical analyses using antibodies against α-tubulin, γ-tubulin, inner centromere protein, Cdc20, Mad2, phospho-histone H3 and cohesin subunits (SMC1α, SMC3 and Rad21) showed that the expression patterns of these proteins in the hybrid embryos are similar to those in the wild-type embryos, except for phospho-histone H3. Phospho-histone H3 present on chromosomes at metaphase was lost from normally separated chromosomes at anaphase, whereas it still existed on lagging chromosomes at anaphase, indicating that the lagging chromosomes remain in the metaphase state even when the cell has proceeded to the anaphase state. On the basis of these findings, we discuss the cellular and molecular mechanisms of chromosome elimination in the hybrid medaka.
Rights: The original publication is available at
Type: article (author version)
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山下 正兼

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