HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
獣医学研究科  >
Japanese Journal of Veterinary Research >
Volume 34, Number 3-4 >

PURIFICATION AND CHARACTERIZATION OF HEMAGGLUTININ OF CLOSTRIDIUM BOTULINUM TYPE C STRAIN STOCKHOLM

フルテキスト
KJ00002374437.pdf563.07 kBPDF見る/開く
この文献には次のDOIがあります:http://doi.org/10.14943/jjvr.34.3-4.269

タイトル: PURIFICATION AND CHARACTERIZATION OF HEMAGGLUTININ OF CLOSTRIDIUM BOTULINUM TYPE C STRAIN STOCKHOLM
著者: SUZUKI, Norihiko 著作を一覧する
SYUTO, Bunei 著作を一覧する
KUBO, Shuichiro 著作を一覧する
キーワード: Clostridium botulinum
hemagglutinin purification
hemagglutinin characterization
発行日: 1986年10月31日
出版者: The Graduate School of Veterinary Medicine
誌名: Japanese Journal of Veterinary Research
巻: 34
号: 3-4
開始ページ: 269
終了ページ: 278
抄録: Clostridium botulinum type C hemagglutinin (HA) was purified and characterized for clarifying the function of HA in botulinum intoxication. The purified HA showed a single band on disc electrophoresis and a single precipitin line on agar gel double diffusion test. The specific activity of the purified HA was 1.6×10^4 units/mg protein for rat erythrocytes. The molecular weight of HA was 230,000 by ultracentrifuge analysis. HA bound not only to erythrocytes but also to intestinal cells. The HA activity was inhibited by the addition of gangliosides (GM_3,GM_4,GD_<1a>, GD_<1b>, and GT_<1b>) and fetuin, and was not shown to erythrocytes treated previously by neuraminidase. These results suggest that HA binds to sialic acid on cell surface. In botulinum intoxication, HA may play as a carrier for the internalization of toxin through small intestine cell.
資料タイプ: bulletin
URI: http://hdl.handle.net/2115/3023
出現コレクション:Volume 34, Number 3-4

 

本サイトに関するご意見・お問い合わせは repo at lib.hokudai.ac.jp へお願いします。 - 北海道大学