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Volume 34, Number 3-4 >

PURIFICATION AND CHARACTERIZATION OF HEMAGGLUTININ OF CLOSTRIDIUM BOTULINUM TYPE C STRAIN STOCKHOLM

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.34.3-4.269

Title: PURIFICATION AND CHARACTERIZATION OF HEMAGGLUTININ OF CLOSTRIDIUM BOTULINUM TYPE C STRAIN STOCKHOLM
Authors: SUZUKI, Norihiko Browse this author
SYUTO, Bunei Browse this author
KUBO, Shuichiro Browse this author
Keywords: Clostridium botulinum
hemagglutinin purification
hemagglutinin characterization
Issue Date: 31-Oct-1986
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 34
Issue: 3-4
Start Page: 269
End Page: 278
Abstract: Clostridium botulinum type C hemagglutinin (HA) was purified and characterized for clarifying the function of HA in botulinum intoxication. The purified HA showed a single band on disc electrophoresis and a single precipitin line on agar gel double diffusion test. The specific activity of the purified HA was 1.6×10^4 units/mg protein for rat erythrocytes. The molecular weight of HA was 230,000 by ultracentrifuge analysis. HA bound not only to erythrocytes but also to intestinal cells. The HA activity was inhibited by the addition of gangliosides (GM_3,GM_4,GD_<1a>, GD_<1b>, and GT_<1b>) and fetuin, and was not shown to erythrocytes treated previously by neuraminidase. These results suggest that HA binds to sialic acid on cell surface. In botulinum intoxication, HA may play as a carrier for the internalization of toxin through small intestine cell.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/3023
Appears in Collections:Japanese Journal of Veterinary Research > Volume 34, Number 3-4

Submitter: 獣医学部図書室

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