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In vivo conversion of triacylglycerol to docosahexaenoic acid-containing phospholipids in a thraustochytrid-like microorganism, strain 12B

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Title: In vivo conversion of triacylglycerol to docosahexaenoic acid-containing phospholipids in a thraustochytrid-like microorganism, strain 12B
Authors: Okuyama, Hidetoshi Browse this author →KAKEN DB
Orikasa, Yoshitake Browse this author
Nishida, Takanori Browse this author
Keywords: Bioconversion
Docosahexaenoic acid (DHA)
DHA-containing phospholipid
Glucose starvation
Thraustochytrid
Issue Date: Dec-2007
Publisher: Springer Netherlands
Journal Title: Biotechnology Letters
Volume: 29
Issue: 12
Start Page: 1977
End Page: 1981
Publisher DOI: 10.1007/s10529-007-9492-5
PMID: 17660943
Abstract: The thraustochytrid-like microorganism, strain 12B, cultivated in peptone, yeast extract, and 8% (w/v) glucose in 50% (v/v) seawater, accumulated docosahexaenoic acid (DHA)-rich triacylglycerol (TAG) at 67% of total lipid. When these TAG-accumulated cells were cultivated in glucose-deficient medium, dry cell weight (3 mg per ml culture) increased approximately 3-fold relative to baseline but the TAG/total lipid decreased to 5%. At the same time, the amount of phospholipid (5 mg) per whole culture also increased 3-fold. Hence, phospholipid/total lipid increased from 13% to 67%. High levels of DHA (more than 50% of total) were maintained in phosphatidylcholine.
Rights: The original publication is available at www.springerlink.com
Type: article (author version)
URI: http://hdl.handle.net/2115/30266
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 奥山 英登志

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