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In vivo conversion of triacylglycerol to docosahexaenoic acid-containing phospholipids in a thraustochytrid-like microorganism, strain 12B
Title: | In vivo conversion of triacylglycerol to docosahexaenoic acid-containing phospholipids in a thraustochytrid-like microorganism, strain 12B |
Authors: | Okuyama, Hidetoshi Browse this author →KAKEN DB | Orikasa, Yoshitake Browse this author | Nishida, Takanori Browse this author |
Keywords: | Bioconversion | Docosahexaenoic acid (DHA) | DHA-containing phospholipid | Glucose starvation | Thraustochytrid |
Issue Date: | Dec-2007 |
Publisher: | Springer Netherlands |
Journal Title: | Biotechnology Letters |
Volume: | 29 |
Issue: | 12 |
Start Page: | 1977 |
End Page: | 1981 |
Publisher DOI: | 10.1007/s10529-007-9492-5 |
PMID: | 17660943 |
Abstract: | The thraustochytrid-like microorganism, strain 12B, cultivated in peptone, yeast extract, and 8% (w/v) glucose in 50% (v/v) seawater, accumulated docosahexaenoic acid (DHA)-rich triacylglycerol (TAG) at 67% of total lipid. When these TAG-accumulated cells were cultivated in glucose-deficient medium, dry cell weight (3 mg per ml culture) increased approximately 3-fold relative to baseline but the TAG/total lipid decreased to 5%. At the same time, the amount of phospholipid (5 mg) per whole culture also increased 3-fold. Hence, phospholipid/total lipid increased from 13% to 67%. High levels of DHA (more than 50% of total) were maintained in phosphatidylcholine. |
Rights: | The original publication is available at www.springerlink.com |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/30266 |
Appears in Collections: | 環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 奥山 英登志
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