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Transbilayer Phospholipid Flipping Regulates Cdc42p Signaling during Polarized Cell Growth via Rga GTPase-Activating Proteins

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Title: Transbilayer Phospholipid Flipping Regulates Cdc42p Signaling during Polarized Cell Growth via Rga GTPase-Activating Proteins
Authors: Saito, Koji Browse this author
Fujimura-Kamada, Konomi Browse this author →KAKEN DB
Hanamatsu, Hisatoshi Browse this author
Kato, Utako Browse this author
Umeda, Masato Browse this author
Kozminski, Keith G. Browse this author
Tanaka, Kazuma Browse this author →KAKEN DB
Keywords: CELLBIO
SIGNALING
Issue Date: 6-Nov-2007
Publisher: Cell Press
Journal Title: Developmental Cell
Volume: 13
Issue: 5
Start Page: 743
End Page: 751
Publisher DOI: 10.1016/j.devcel.2007.09.014
PMID: 17981141
Abstract: An important problem in polarized morphogenesis is how polarized transport of membrane vesicles is spatiotemporally regulated. Here, we report that a local change in the transbilayer phospholipid distribution of the plasma membrane regulates the axis of polarized growth. Type 4 P-type ATPases Lem3p-Dnf1p and -Dnf2p are putative heteromeric phospholipid flippases in budding yeast that are localized to polarized sites on the plasma membrane. The lem3Δ mutant exhibits prolonged apical growth due to a defect in the switch to isotropic bud growth. In lem3Δ cells, the small GTPase Cdc42p remains polarized at the bud tip where phosphatidylethanolamine remains exposed on the outer leaflet. Intriguingly, phosphatidylethanolamine and phosphatidylserine stimulate GTPase-activating protein (GAP) activity of Rga1p and Rga2p toward Cdc42p, whereas PI(4,5)P2 inhibits it. We propose that a redistribution of phospholipids to the inner leaflet of the plasma membrane triggers the dispersal of Cdc42p from the apical growth site, through activation of GAPs.
Relation: http://www.developmentalcell.com/
Type: article (author version)
URI: http://hdl.handle.net/2115/30299
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 田中 一馬

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