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A new primer set for sex identification in the genus Sorex (Soricidae, Insectivora)

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Title: A new primer set for sex identification in the genus Sorex (Soricidae, Insectivora)
Authors: Matsubara, K. Browse this author
Ishibashi, Y. Browse this author
Ohdachi, S. Browse this author →KAKEN DB
Matsuda, Y. Browse this author
Keywords: PCR
sex identification
shrew
Sorex
Soricidae
SRY HMG box
Issue Date: Dec-2001
Publisher: Blackwell Publishing
Journal Title: Molecular Ecology Notes
Volume: 1
Issue: 4
Start Page: 241
End Page: 242
Publisher DOI: 10.1046/j.1471-8278.2001.00089.x
Abstract: In order to develop a new accurate method for sexing in Sorex species (Soricidae, Insectivora), we synthesized a polymerase chain reaction (PCR) primer set to amplify a part of Sry HMG box in the long-clawed shrew, Sorex unguiculatus. When the primers were applied to the samples of known sex, PCR products were successfully obtained for males as a clear, single band on 3% agarose gels after electrophoresis in Sorex unguiculatus and five other Sorex species, but not for females of these six species. Thus, PCR amplification using the primer set may be applicable to discern sex in the six Sorex species.
Rights: The definitive version is available at www.blackwell-synergy.com
Type: article (author version)
URI: http://hdl.handle.net/2115/30314
Appears in Collections:創成研究機構 (Creative Research Institution) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 松原 和純

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