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Purification of multiple vitellogenins in grey mullet (Mugil cephalus)

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タイトル: Purification of multiple vitellogenins in grey mullet (Mugil cephalus)
著者: Amano, Haruna 著作を一覧する
Fujita, Toshiaki 著作を一覧する
Hiramatsu, Naoshi 著作を一覧する
Sawaguchi, Sayumi 著作を一覧する
Matsubara, Takahiro 著作を一覧する
Sullivan, Craig V 著作を一覧する
Hara, Akihiko 著作を一覧する
発行日: 2007年11月
出版者: Springer
誌名: Marine Biology
巻: 152
号: 6
開始ページ: 1215
終了ページ: 1225
出版社 DOI: 10.1007/s00227-007-0768-z
抄録: Three female specific serum proteins were detected immunologically in the sera of grey mullet (Mugil cephalus) which were named vitellogenin A (VgA), VgB, and VgC, based upon their distinct antigenicity against specific antisera raised against three types of mullet lipovitellins (Lvs). These Vgs were subsequently purified from the serum of estradiol-treated mullet by combining several types of chromatography columns (anion exchanger, hydroxylapatite, immunoadsorbent column, and gel filtration). Purified native VgA, VgB, and VgC exhibited molecular masses of 570, 580, and 335 kDa, respectively. Following, SDS-PAGE, the estimated mass of polypeptide bands evident for VgA and VgB were ~179 kDa and ~175 kDa, respectively; VgC appeared to be ~132 kDa. The two larger Vgs (VgA and VgB) appeared to be phosphorylated, suggesting that these Vgs contain a highly phosphorylated, serine-rich phosvitin (Pv) domain. Furthermore, two discrete Vg-type specific antisera, anti-VgA and anti-VgB, were developed and each generated two precipitin lines against ovary extracts in immunoelectrophoresis, indicating that these Vgs contain additional antigenic yolk protein domains: Lv and β'-component. The small Vg (VgC) appeared to lack a Pv domain because of its low serine content (5.35%) and failure to show positive results in phospho-staining experiments. In conjunction with N-terminal amino acid sequencing analyses of the purified Vgs, our present results have conclusively identified the purified Vg products in grey mullet as typical A-type (VgA), B-type (VgB), and C-type (VgC) Vgs.
Rights: The original publication is available at www.springerlink.com
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/30349
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 原 彰彦

 

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