HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Japanese Journal of Veterinary Research >
Volume 37, Number 2 >

USE OF BIOTINYLATED ANTIBODY FOR THE ASSAY OF HANGANUTZIU-DEICHER ANTIBODIES AND ANTIGENS IN FLUIDS AND TISSUES FROM CANCER PATIENTS

Files in This Item:
KJ00002377235.pdf904.22 kBPDFView/Open
Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.37.2.71

Title: USE OF BIOTINYLATED ANTIBODY FOR THE ASSAY OF HANGANUTZIU-DEICHER ANTIBODIES AND ANTIGENS IN FLUIDS AND TISSUES FROM CANCER PATIENTS
Authors: GATHURU, John K. Browse this author
HIGASHI, Hideyoshi Browse this author
KATO, Shiro Browse this author
USUBA, Osamu Browse this author
NAIKI, Masaharu Browse this author
Keywords: ELISA
Hanganutziu-Deicher antibodies ; Tumor-associated antigens
Issue Date: 20-Jun-1989
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 37
Issue: 2
Start Page: 71
End Page: 83
Abstract: An improved enzyme-linked immunosorbent assay (ELISA) for detection of heterophile Hanganutziu-Deicher (HD) antibodies and antigens, which are frequently detected in sera and/or cancerous tissues from patients with various cancers was developed using biotinylated chicken anti-GM3(NeuGc) antibody and avidin-horse raddish peroxidase conjugate. The N-glycolylneuraminyllactosyl-ceramide, GM3(NeuGc) ganglioside was purified from horse erythrocyte memberanes. The ELISA proceduure required 300 ng GM3(NeuGc) antigen to coat plastic microtiter plates and 190 ng biotinylated antibody per well to give optimum product formation. The technique could detect 6 ng antigen in tissue homogenate as compared to 0.6 ng of the pure compound by inhibition. Chicken anti-GM3(NeuGc) antibody quantitatively inhibited the biotinylated antibody, however, this procedure was not suitable to quantify lower affinity HD antibody in patient sera. Immunostaining specific for HD antigen-positive cells, in tissue sections was by 4 μg/ml biotinylted antibody and 200 dilution of Avidin-biotinylated peroxidase complex reagent using pig intestine and lymph node as positive tissues and chicken intestine and lung as negative tissues.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/3142
Appears in Collections:Japanese Journal of Veterinary Research > Volume 37, Number 2

Submitter: 獣医学部図書室

Export metadata:

OAI-PMH ( junii2 , jpcoar )


 

Feedback - Hokkaido University