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Volume 37, Number 3-4 >

CYTOTOXIC EFFECT OF ACYCLOVIR ON CULTURED MAMMALIAN CELLS TO WHICH HERPESVIRUS THYMIDINE KINASE GENE WAS INTRODUCED

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.37.3-4.181

Title: CYTOTOXIC EFFECT OF ACYCLOVIR ON CULTURED MAMMALIAN CELLS TO WHICH HERPESVIRUS THYMIDINE KINASE GENE WAS INTRODUCED
Authors: TANABE, Kiyoshi Browse this author
HIRAOKA, Wakako Browse this author
KUWABARA, Mikinori Browse this author
SATO, Fumiaki Browse this author
NARITA, Takahiro Browse this author
NIIKURA, Masahiro Browse this author
Keywords: acyclovir
herpesvirus thymidine kinase
cytotoxicity
phosphorylation
gene transfer
Issue Date: 10-Nov-1989
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 37
Issue: 3-4
Start Page: 181
End Page: 185
Abstract: The cytotoxic effects of acyclovir, which is a purine nucleoside analogue and is known as an antibiotic substance, were examined on three lines of rat skin fibroblast FR cells ; normal FR cells, FRtk^- cells which are deficient in the activity of thymidine kinase (tk) and FRtk^-HSVtk^+ cells which were prepared by introducing herpes simplex virus' tk gene to FRtk^- cells. When FRtk^-HSVtk^+ cells growing exponentially were incubated in the presence of acyclovir for 4 h, the surviving fractions of the cells decreased in a concertration-dependent manner. Whereas, decrease of the surviving fractions was almost indiscernible in both FR cells and FRtk^- cells at the whole ranges of drug-concentrations tested. These results indicate that acyclovir is phosphorylated by the herpes simplex virus' tk and becomes toxic to FRtk^-HSVtk^+ cells. This also means that FRtk^-HSVtk^+ cells are useful for the investigation of the biological activity of nucleoside analogues.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/3189
Appears in Collections:Japanese Journal of Veterinary Research > Volume 37, Number 3-4

Submitter: 獣医学部図書室

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