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Volume 37, Number 3-4 >

CYTOTOXIC EFFECT OF ACYCLOVIR ON CULTURED MAMMALIAN CELLS TO WHICH HERPESVIRUS THYMIDINE KINASE GENE WAS INTRODUCED

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この文献には次のDOIがあります:http://doi.org/10.14943/jjvr.37.3-4.181

タイトル: CYTOTOXIC EFFECT OF ACYCLOVIR ON CULTURED MAMMALIAN CELLS TO WHICH HERPESVIRUS THYMIDINE KINASE GENE WAS INTRODUCED
著者: TANABE, Kiyoshi 著作を一覧する
HIRAOKA, Wakako 著作を一覧する
KUWABARA, Mikinori 著作を一覧する
SATO, Fumiaki 著作を一覧する
NARITA, Takahiro 著作を一覧する
NIIKURA, Masahiro 著作を一覧する
キーワード: acyclovir
herpesvirus thymidine kinase
cytotoxicity
phosphorylation
gene transfer
発行日: 1989年11月10日
出版者: The Graduate School of Veterinary Medicine
誌名: Japanese Journal of Veterinary Research
巻: 37
号: 3-4
開始ページ: 181
終了ページ: 185
抄録: The cytotoxic effects of acyclovir, which is a purine nucleoside analogue and is known as an antibiotic substance, were examined on three lines of rat skin fibroblast FR cells ; normal FR cells, FRtk^- cells which are deficient in the activity of thymidine kinase (tk) and FRtk^-HSVtk^+ cells which were prepared by introducing herpes simplex virus' tk gene to FRtk^- cells. When FRtk^-HSVtk^+ cells growing exponentially were incubated in the presence of acyclovir for 4 h, the surviving fractions of the cells decreased in a concertration-dependent manner. Whereas, decrease of the surviving fractions was almost indiscernible in both FR cells and FRtk^- cells at the whole ranges of drug-concentrations tested. These results indicate that acyclovir is phosphorylated by the herpes simplex virus' tk and becomes toxic to FRtk^-HSVtk^+ cells. This also means that FRtk^-HSVtk^+ cells are useful for the investigation of the biological activity of nucleoside analogues.
資料タイプ: bulletin
URI: http://hdl.handle.net/2115/3189
出現コレクション:Volume 37, Number 3-4

 

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