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Volume 38, Number 2 >

RESTRICTION FRAGMENT LENGTH POLYMORPHISM FOR THE YC SUBUNIT GENE OF RAT LIVER GLUTATHIONE S-TRANSFERASE

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.38.2.35

Title: RESTRICTION FRAGMENT LENGTH POLYMORPHISM FOR THE YC SUBUNIT GENE OF RAT LIVER GLUTATHIONE S-TRANSFERASE
Authors: SASAKI, Yasuhiko Browse this author
HAYASHI, Masanobu Browse this author
MATSUMOTO, Kouzou Browse this author
NAMIOKA, Shigeo Browse this author
Keywords: RFLP
Yc subunit gene
rat liver GST
Issue Date: 20-Jul-1990
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 38
Issue: 2
Start Page: 35
End Page: 42
Description: An altered expression of the Yc subunit gene of rat glutathione S-transferase (GST) in the liver of the LEC rat, which is a mutant strain with spontaneous hereditary hepatitis associated with severe jaundice, has been reported. To provide further information concerning the structure of the Yc subunit gene, we carried out the Southern blot hybridization analysis of DNA samples from rats of eight different inbred strains including LEC with cDNA complementary to mRNA specific for the Yc subunit of rat liver GST as a probe. The hybridization patterns of the DNA samples from rats belonging to the different inbred strains showed interstrain variation in the length of restriction fragments with four restriction endonucleases. Since the DNA samples prepared from several rats of one inbred strain gave an identical hybridization pattern, the restriction fragment patterns for the Yc gene could be used as markers for genetic monitoring of inbred rat strains. Although the altered expression of Yc-Yc activity of GST has been observed in the liver of the LEC rat, the characteristic changes in the gene structure of the Yc subunit of LEC rat were not detected in the present hybridization analysis.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/3193
Appears in Collections:Japanese Journal of Veterinary Research > Volume 38, Number 2

Submitter: 獣医学部図書室

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