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Ecdysone response element in a baculovirus immediate-early gene, ie1, promoter

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Title: Ecdysone response element in a baculovirus immediate-early gene, ie1, promoter
Authors: Kojima, K. Browse this author
Oritani, K. Browse this author
Nakatsukasa, T. Browse this author
Asano, S. Browse this author
Sahara, K. Browse this author →KAKEN DB
Bando, H. Browse this author →KAKEN DB
Keywords: BmNPV
immediate-early 1
Issue Date: 2007
Publisher: Elsevier
Journal Title: Virus Research
Volume: 130
Issue: 1-2
Start Page: 202
End Page: 209
Publisher DOI: 10.1016/j.virusres.2007.06.012
PMID: 17658648
Abstract: A computer-assisted analysis identified tentative target sequences for regulatory proteins including ecdysone-inducible factors such as BmFTZF1 and Broad-Complex Z4 (BR-C Z4) in the ie1 promoter of BmNPV. A transient expression experiment using BmN cells and a series of truncated ie1 promoter constructs demonstrated that the activity of the ie1 promoter responded to alpha-ecdysone and 20-hydroxyecdysone, which required a tridecameric nucleotide stretch (ie1EcRE, 5'-GTGTTATCGACCT-3') homologous to the ecdysone response element reported for Drosophila (DmEcRE). RT-PCR demonstrated the expression of BmEcR and BmUSP, which are required as ecdysone-specific activators for EcRE-mediated activation, in BmN cells. Furthermore, the ie1 EcRE-mediated response was confirmed by using a recombinant BmNPV possessing a luciferase gene under the control of the ie1 promoter with or without ie1 EcRE. This is the first report of an ecdysone response element in a baculoviral gene promoter. These results also suggested that the regulation of the iel by ecdysone may militate viral replication at least under certain conditions during natural infections in vivo.
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 伴戸 久徳

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