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Hokkaido University Collection of Scholarly and Academic Papers >
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Ecdysone response element in a baculovirus immediate-early gene, ie1, promoter
| Title: | Ecdysone response element in a baculovirus immediate-early gene, ie1, promoter |
| Authors: | Kojima, K. Browse this author | | Oritani, K. Browse this author | | Nakatsukasa, T. Browse this author | | Asano, S. Browse this author | | Sahara, K. Browse this author →KAKEN DB | | Bando, H. Browse this author →KAKEN DB |
| Keywords: | BmNPV | | immediate-early 1 | | ecdysteroids |
| Issue Date: | 2007 |
| Publisher: | Elsevier |
| Journal Title: | Virus Research |
| Volume: | 130 |
| Issue: | 1-2 |
| Start Page: | 202 |
| End Page: | 209 |
| Publisher DOI: | 10.1016/j.virusres.2007.06.012 |
| PMID: | 17658648 |
| Abstract: | A computer-assisted analysis identified tentative target sequences for regulatory proteins including ecdysone-inducible factors such as BmFTZF1 and Broad-Complex Z4 (BR-C Z4) in the ie1 promoter of BmNPV. A transient expression experiment using BmN cells and a series of truncated ie1 promoter constructs demonstrated that the activity of the ie1 promoter responded to alpha-ecdysone and 20-hydroxyecdysone, which required a tridecameric nucleotide stretch (ie1EcRE, 5'-GTGTTATCGACCT-3') homologous to the ecdysone response element reported for Drosophila (DmEcRE). RT-PCR demonstrated the expression of BmEcR and BmUSP, which are required as ecdysone-specific activators for EcRE-mediated activation, in BmN cells. Furthermore, the ie1 EcRE-mediated response was confirmed by using a recombinant BmNPV possessing a luciferase gene under the control of the ie1 promoter with or without ie1 EcRE. This is the first report of an ecdysone response element in a baculoviral gene promoter. These results also suggested that the regulation of the iel by ecdysone may militate viral replication at least under certain conditions during natural infections in vivo. |
| Relation: | http://www.sciencedirect.com/science/journal/01681702 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/32318 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 伴戸 久徳
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