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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Medicine / Faculty of Medicine >
Peer-reviewed Journal Articles, etc >
Ubiquitylation of epsilon-COP by PIRH2 and regulation of the secretion of PSA
| Title: | Ubiquitylation of epsilon-COP by PIRH2 and regulation of the secretion of PSA |
| Authors: | Maruyama, Satoru Browse this author | | Miyajima, Naoto Browse this author | | Bohgaki, Miyuki Browse this author | | Tsukiyama, Tadasuke Browse this author | | Shigemura, Masahiko Browse this author | | Nonomura, Katsuya Browse this author | | Hatakeyama, Shigetsugu Browse this author →KAKEN DB |
| Keywords: | PIRH2 | | ε-COP | | ubiquitin | | androgen receptor | | PSA |
| Issue Date: | Jan-2008 |
| Publisher: | Springer |
| Journal Title: | Molecular and Cellular Biochemistry |
| Volume: | 307 |
| Issue: | 1-2 |
| Start Page: | 73 |
| End Page: | 82 |
| Publisher DOI: | 10.1007/s11010-007-9586-3 |
| PMID: | 17721809 |
| Abstract: | Ubiquitylation appears to be involved in the membrane trafficking system including endocytosis, exocytosis, and ER-to-Golgi transport. We found that PIRH2, which was identified as an interacting protein for androgen receptor or p53, interacts with and ubiquitylates the ε-subunit of coatmer complex, ε-COP. PIRH2 promotes the ubiquitylation of ε-COP in vitro and in vivo and consequently promotes the degradation of ε-COP. The interaction between PIRH2 and ε-COP is affected by the presence of androgen, and PIRH2 in the presence of androgen promotes ubiquitylation of ε-COP in vivo. Furthermore, overexpression of the wild type of PIRH2 in prostate cancer cells causes downregulation of the secretion of prostate-specific antigen (PSA), a secretory protein in prostate epithelial cells and one of diagnostic markers for prostate cancer. Our results indicate that PIRH2 functions as a regulator for COP I complex. |
| Rights: | The original publication is available at www.springerlink.com |
| Relation: | http://www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/32324 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 畠山 鎮次
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