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Volume 38, Number 3-4 >

TIMING OF SEQUENTIAL CHANGES IN CHROMOSOME CONFIGURATIONS DURING THE 1st MEIOTIC DIVISION OF PIG OOCYTES CULTURED IN VITRO

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.38.3-4.127

Title: TIMING OF SEQUENTIAL CHANGES IN CHROMOSOME CONFIGURATIONS DURING THE 1st MEIOTIC DIVISION OF PIG OOCYTES CULTURED IN VITRO
Authors: OCAMPO, Marlon B. Browse this author
OCAMPO, Lerma T.C. Browse this author
KANAGAWA, Hiroshi Browse this author
Keywords: 1st meiosis
chromosome
pig oocytes
in vitro
Issue Date: 28-Dec-1990
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 38
Issue: 3-4
Start Page: 127
End Page: 137
Abstract: This study examines the timing of changes in chromosome configurations of pig oocytes derived from small antral follicles of follicular and/or inactive stage donors using modified 199 medium supplemented with gonadotropins (Follicle Stimulating Hormone (FSH), 10 IU/ml ; Human Chorionic Gonadotropin (hCG), 10 IU/ml) and Glucosamine (0.539mg/ml). Oocytes (n=1,215) were fixed at the end of 3 hourly intervals from 0-48 hr of culture. Results were expressed as the percentage of oocytes at each stage of maturation for each time point. The germinal vesicle (GV) stage was observed for the first 17.6 hr ; germinal vesicle breakdown (GVBD) stage between 17.6-26.4 hr ; metaphase I (M-I) from 26.4-30.9 hr ; anaphase I (A-I) ranged from 30.9-33.4 hr ; telophase I (T-I) at 33.4-34.4 hr ; and metaphase II (M-II) at 34.4-48 hr.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/3235
Appears in Collections:Japanese Journal of Veterinary Research > Volume 38, Number 3-4

Submitter: 獣医学部図書室

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