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Ultrasensitive detection of organophosphate insecticides by carbon nanotube field-effect transistor

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Title: Ultrasensitive detection of organophosphate insecticides by carbon nanotube field-effect transistor
Authors: Ishii, Atsushi Browse this author
Takeda, Seiji Browse this author
Hattori, Satoshi Browse this author
Sueoka, Kazuhisa Browse this author
Mukasa, Koichi Browse this author
Keywords: field-effect transistor
Issue Date: 1-Feb-2007
Publisher: Elsevier
Journal Title: Colloids and Surfaces A Physicochemical and Engineering Aspects
Volume: 313
Start Page: 456
End Page: 460
Publisher DOI: 10.1016/j.colsurfa.2007.05.071
Abstract: Carbon nanotube sensors were capable of detecting organophosphate insecticide binding to recombinant acetylcholinesterase (rAChE) immobilized on the sensor. The carbon nanotube sensors were fabricated by chemical vapor deposition method and it showed field-effect transistor property. rAChE was immobilized by specific binding of NTA-Ni and His tag on the reverse side of the carbon nanotube sensor. The current between the source and drain was measured after incubation of various concentrations of organophosphates with immobilized rAChE. I-ds-V-gate curves were obtained by plotting the current as a function of the potential applied to the back gate. The I-ds-V-gate curves showed a positive shift in a dose dependent manner, while no shifts were observed when an herbicide was added to the sensor and organophosphates were withdrawn on recombinant calmodulin immobilized surface. In spite of different inhibition effects, two organophosphates showed similar dose response curves in measuring I-ds-V-gate correlation with the rAChE-immobilized CNT sensors, suggesting that the binding of organophosphates to rAChE may be sequestered from the inhibition activity of those to the enzyme.
Type: article (author version)
Appears in Collections:創成研究機構 (Creative Research Institution) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 石井 睦

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