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Ultrasensitive detection of organophosphate insecticides by carbon nanotube field-effect transistor
| Title: | Ultrasensitive detection of organophosphate insecticides by carbon nanotube field-effect transistor |
| Authors: | Ishii, Atsushi Browse this author | | Takeda, Seiji Browse this author | | Hattori, Satoshi Browse this author | | Sueoka, Kazuhisa Browse this author | | Mukasa, Koichi Browse this author |
| Keywords: | field-effect transistor | | biochemistry | | biosensors |
| Issue Date: | 1-Feb-2007 |
| Publisher: | Elsevier |
| Journal Title: | Colloids and Surfaces A Physicochemical and Engineering Aspects |
| Volume: | 313 |
| Start Page: | 456 |
| End Page: | 460 |
| Publisher DOI: | 10.1016/j.colsurfa.2007.05.071 |
| Abstract: | Carbon nanotube sensors were capable of detecting organophosphate insecticide binding to recombinant acetylcholinesterase (rAChE) immobilized on the sensor. The carbon nanotube sensors were fabricated by chemical vapor deposition method and it showed field-effect transistor property. rAChE was immobilized by specific binding of NTA-Ni and His tag on the reverse side of the carbon nanotube sensor. The current between the source and drain was measured after incubation of various concentrations of organophosphates with immobilized rAChE. I-ds-V-gate curves were obtained by plotting the current as a function of the potential applied to the back gate. The I-ds-V-gate curves showed a positive shift in a dose dependent manner, while no shifts were observed when an herbicide was added to the sensor and organophosphates were withdrawn on recombinant calmodulin immobilized surface. In spite of different inhibition effects, two organophosphates showed similar dose response curves in measuring I-ds-V-gate correlation with the rAChE-immobilized CNT sensors, suggesting that the binding of organophosphates to rAChE may be sequestered from the inhibition activity of those to the enzyme. |
| Relation: | http://www.sciencedirect.com/science/journal/09277757 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/32365 |
| Appears in Collections: | 創成研究機構 (Creative Research Institution) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 石井 睦
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