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Volume 39, Number 1 >

PMSG PROFILES IN SUPEROVULATED AND ANTI-PMSG ANTISERUM TREATED MICE AND HEIFERS WITH ENZYMEIMMUNOASSAY

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.39.1.11

Title: PMSG PROFILES IN SUPEROVULATED AND ANTI-PMSG ANTISERUM TREATED MICE AND HEIFERS WITH ENZYMEIMMUNOASSAY
Authors: KATAGIRI, Seiji Browse this author
TAKAHASHI, Yoshiyuki Browse this author
HISHINUMA, Mitsugu Browse this author
KANAGAWA, Hiroshi Browse this author
DOCHI, Osamu Browse this author
TAKAKURA, Hirosuke Browse this author
Keywords: PMSG
anti-PMSG
superovulation
mouse
heifer
Issue Date: 30-May-1991
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 39
Issue: 1
Start Page: 11
End Page: 21
Abstract: A sandwich enzymeimmunoassay (EIA) for pregnant mare serum gonadotropin (PMSG) using a microtiter plate was developed. Sensitivity of the assay to PMSG was 15.6 mIU/ml (0.2 ng/well). The PMSG levels in serum were measured with the EIA in superovulated and anti-PMSG rabbit antiserum treated mice and heifers. In mice, the PMSG blood level was measurable in the serum 4-6 days after intraperitoneal injection of 5-30 IU of PMSG. The administration of anti-PMSG antiserum at the same dose level as PMSG caused a rapid decrease in the PMSG blood level, declining to undetectable levels within 17 hours. In heifers, the PMSG level was measurable at 10-11 days after the injection of 2500 or 3000 IU of PMSG. When antiserum was injected 48 hours after the PMSG injection, the clearance rate of PMSG was affected by the route of the administration. The administration of 3000 units of anti-PMSG antiserum intravenously caused a rapid decline and the disappearance of circulating PMSG within 17 hours. When 3000 units of anti-PMSG antiserum was injected intramuscularly, the PMSG blood level also decreased and became unmeasurable 24 hours after administration ; however, it was still detectable for up to 17 hours. These results indicate that the administration of anti-PMSG antiserum at the proper timing and dosage could lead to successful superovulation through the improvement of hormonal conditions.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/3238
Appears in Collections:Japanese Journal of Veterinary Research > Volume 39, Number 1

Submitter: 片桐 成二

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