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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
北海道大学水産科学研究彙報 = Bulletin of Fisheries Sciences, Hokkaido University >
第57巻 第3号 >
Regiospecific Analysis of Marine Oil Triacylglycerols Using Boric Acid High-Performance Thin-Layer Chromatography
| Title: | Regiospecific Analysis of Marine Oil Triacylglycerols Using Boric Acid High-Performance Thin-Layer Chromatography |
| Authors: | Ando, Yasuhiro Browse this author →KAKEN DB | | Haba, Yusuke Browse this author | | Takase, Kiwamu Browse this author | | Sakai, Shinya Browse this author |
| Keywords: | Regiospecific analysis | | High-performance thin-layer chromatography | | Triacylglycerol | | Fatty acid | | Fish oil | | Seal oil |
| Issue Date: | Dec-2007 |
| Publisher: | 北海道大学大学院水産科学研究院 |
| Journal Title: | 北海道大学水産科学研究彙報 |
| Journal Title(alt): | BULLETIN OF FISHERIES SCIENCES, HOKKAIDO UNIVERSITY |
| Volume: | 57 |
| Issue: | 3 |
| Start Page: | 37 |
| End Page: | 41 |
| Abstract: | This paper presents a smaller-sized procedure for regiospecific analysis of triacylglycerols (TAG) using boric acid high-performance thin-layer chromatography (HPTLC). Cod liver/mackerel, bonito head, and seal oils TAG (2mg) were partially hydrolyzed by ethyl magnesium bromide, and resulting 1(3)-and 2-monoacylglycerols (MAG) were isolated by the HPTLC. Fatty acids of the 1(3)-and 2-MAG were analyzed by gas-liquid chromatography (GLC). Positional distributions of fatty acids in TAG observed for the three marine oils were similar to those previously determined by the procedure requiring 100mg of starting TAG. The new procedure appeared to be usable for regiospecific analysis of a few milligrams of marine oils TAG. |
| Type: | bulletin (article) |
| URI: | http://hdl.handle.net/2115/32486 |
| Appears in Collections: | 北海道大学水産科学研究彙報 = Bulletin of Fisheries Sciences, Hokkaido University > 第57巻 第3号
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