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北海道大学水産科学研究彙報 = Bulletin of Fisheries Sciences, Hokkaido University >
第57巻 第3号 >

Regiospecific Analysis of Marine Oil Triacylglycerols Using Boric Acid High-Performance Thin-Layer Chromatography

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Title: Regiospecific Analysis of Marine Oil Triacylglycerols Using Boric Acid High-Performance Thin-Layer Chromatography
Authors: Ando, Yasuhiro Browse this author →KAKEN DB
Haba, Yusuke Browse this author
Takase, Kiwamu Browse this author
Sakai, Shinya Browse this author
Keywords: Regiospecific analysis
High-performance thin-layer chromatography
Triacylglycerol
Fatty acid
Fish oil
Seal oil
Issue Date: Dec-2007
Publisher: 北海道大学大学院水産科学研究院
Journal Title: 北海道大学水産科学研究彙報
Journal Title(alt): BULLETIN OF FISHERIES SCIENCES, HOKKAIDO UNIVERSITY
Volume: 57
Issue: 3
Start Page: 37
End Page: 41
Abstract: This paper presents a smaller-sized procedure for regiospecific analysis of triacylglycerols (TAG) using boric acid high-performance thin-layer chromatography (HPTLC). Cod liver/mackerel, bonito head, and seal oils TAG (2mg) were partially hydrolyzed by ethyl magnesium bromide, and resulting 1(3)-and 2-monoacylglycerols (MAG) were isolated by the HPTLC. Fatty acids of the 1(3)-and 2-MAG were analyzed by gas-liquid chromatography (GLC). Positional distributions of fatty acids in TAG observed for the three marine oils were similar to those previously determined by the procedure requiring 100mg of starting TAG. The new procedure appeared to be usable for regiospecific analysis of a few milligrams of marine oils TAG.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/32486
Appears in Collections:北海道大学水産科学研究彙報 = Bulletin of Fisheries Sciences, Hokkaido University > 第57巻 第3号

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