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Effect of honeycomb film on protein adsorption, cell adhesion and proliferation
| Title: | Effect of honeycomb film on protein adsorption, cell adhesion and proliferation |
| Authors: | Sunami, Hiroshi Browse this author | | Ito, Emiko Browse this author | | Tanaka, Masaru Browse this author | | Yamamoto, Sadaaki Browse this author | | Shimomura, Masatsugu Browse this author |
| Keywords: | Honeycomb film | | Cell adhesion | | Cell proliferation | | Fibronectin | | Tissue engineering | | Self-organization |
| Issue Date: | 15-Aug-2006 |
| Publisher: | Elsevier Science |
| Journal Title: | Colloids and Surfaces. A, Physicochemical and Engineering Aspects |
| Volume: | 284-285 |
| Start Page: | 548 |
| End Page: | 551 |
| Publisher DOI: | 10.1016/j.colsurfa.2005.11.041 |
| Abstract: | This article describes novel methods for controlling of cell adhesion by using micro porous polymer films. Recently we found the highly ordered micro porous films were formed when poly(ε-caprolactone)(PCL) solution was cast on substrates at high atmospheric humidity. The micro porous film has regular honeycomb morphology with a size of 5 μm per cell (honeycomb film). Endothelial cells grew rapidly on the honeycomb film. After 24 h cell culture, the cell number on honeycomb films was lager than that on PCL flat films. In order to elucidate the effect of honeycomb films as a scaffold for cell culture, the adsorbed proteins on honeycomb films under cell culture condition were observed. After conditioning of the honeycomb film and the flat film in DMEM containing 10 % foetal bovine serum (FBS) for 72 h at 37 ℃ in 5% CO2 atmosphere, the adsorbed fibronectin-FITC and albumin-Texasred on the honeycomb films was observed by using confocal laser scanning microscope (CLSM). The observation revealed that fibronectin showed site-selective adsorption behavior on the honeycomb film. Albumin adsorbed on the honeycomb film non site-selectively, while fibronectin mainly adsorbed on inside of honeycomb pores. On the flat film, fibronectin was hardly observed. Since the honeycomb film accelerates the adsorption of fibronectin which is a typical protein as a cell adhesion molecule, the film could be a scaffold with excellent cell adhesion properties. |
| Relation: | http://www.sciencedirect.com/science/journal/09277757 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/32751 |
| Appears in Collections: | 創成研究機構 (Creative Research Institution) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 角南 寛
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