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Effect of honeycomb film on protein adsorption, cell adhesion and proliferation

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Title: Effect of honeycomb film on protein adsorption, cell adhesion and proliferation
Authors: Sunami, Hiroshi Browse this author
Ito, Emiko Browse this author
Tanaka, Masaru Browse this author
Yamamoto, Sadaaki Browse this author
Shimomura, Masatsugu Browse this author
Keywords: Honeycomb film
Cell adhesion
Cell proliferation
Tissue engineering
Issue Date: 15-Aug-2006
Publisher: Elsevier Science
Journal Title: Colloids and Surfaces. A, Physicochemical and Engineering Aspects
Volume: 284-285
Start Page: 548
End Page: 551
Publisher DOI: 10.1016/j.colsurfa.2005.11.041
Abstract: This article describes novel methods for controlling of cell adhesion by using micro porous polymer films. Recently we found the highly ordered micro porous films were formed when poly(ε-caprolactone)(PCL) solution was cast on substrates at high atmospheric humidity. The micro porous film has regular honeycomb morphology with a size of 5 μm per cell (honeycomb film). Endothelial cells grew rapidly on the honeycomb film. After 24 h cell culture, the cell number on honeycomb films was lager than that on PCL flat films. In order to elucidate the effect of honeycomb films as a scaffold for cell culture, the adsorbed proteins on honeycomb films under cell culture condition were observed. After conditioning of the honeycomb film and the flat film in DMEM containing 10 % foetal bovine serum (FBS) for 72 h at 37 ℃ in 5% CO2 atmosphere, the adsorbed fibronectin-FITC and albumin-Texasred on the honeycomb films was observed by using confocal laser scanning microscope (CLSM). The observation revealed that fibronectin showed site-selective adsorption behavior on the honeycomb film. Albumin adsorbed on the honeycomb film non site-selectively, while fibronectin mainly adsorbed on inside of honeycomb pores. On the flat film, fibronectin was hardly observed. Since the honeycomb film accelerates the adsorption of fibronectin which is a typical protein as a cell adhesion molecule, the film could be a scaffold with excellent cell adhesion properties.
Type: article (author version)
Appears in Collections:創成研究機構 (Creative Research Institution) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 角南 寛

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