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Radiation-induced apoptosis of tumor cells is facilitated by inhibition of the interaction between Survivin and Smac/DIABLO

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/32876

Title: Radiation-induced apoptosis of tumor cells is facilitated by inhibition of the interaction between Survivin and Smac/DIABLO
Authors: Ogura, Aki Browse this author
Watanabe, Yasuko Browse this author
Iizuka, Daisuke Browse this author
Yasui, Hironobu Browse this author →KAKEN DB
Amitani, Makoto Browse this author
Kobayashi, Saori Browse this author
Kuwabara, Mikinori Browse this author
Inanami, Osamu Browse this author →KAKEN DB
Keywords: Survivin
radiation
apoptosis
dominant negative mutant
adenoviral vector
Issue Date: 18-Jan-2008
Publisher: Elsevier
Journal Title: Cancer Letters
Volume: 259
Issue: 1
Start Page: 71
End Page: 81
Publisher DOI: 10.1016/j.canlet.2007.09.017
PMID: 17967504
Abstract: To investigate the mechanism of radioresistance of solid tumor cells, we created two expression vectors encoding Survivin mutants, T34A and D53A. When T34A and D53A were overexpressed in NIH3T3, A549 and HeLa cells, radiation-induced apoptosis was significantly enhanced. Furthermore, we examined the binding capability of Survivin with Smac/DIABLO in the cells that overexpressed these mutants. Coimmunoprecipitation analysis revealed that mutant form of Survivin, D53A and T34A could bind to Smac/DIABLO, but with much less affinity compared to the authentic form. These results suggest that radiation-induced apoptosis of tumor cells is increased by inhibition of the interaction between Survivin and Smac/DIABLO through overexpression of T34A and D53A.
Relation: http://www.sciencedirect.com/science/journal/03043835
Type: article (author version)
URI: http://hdl.handle.net/2115/32876
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 稲波 修

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