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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Radiation-induced apoptosis of tumor cells is facilitated by inhibition of the interaction between Survivin and Smac/DIABLO
| Title: | Radiation-induced apoptosis of tumor cells is facilitated by inhibition of the interaction between Survivin and Smac/DIABLO |
| Authors: | Ogura, Aki Browse this author | | Watanabe, Yasuko Browse this author | | Iizuka, Daisuke Browse this author | | Yasui, Hironobu Browse this author →KAKEN DB | | Amitani, Makoto Browse this author | | Kobayashi, Saori Browse this author | | Kuwabara, Mikinori Browse this author | | Inanami, Osamu Browse this author →KAKEN DB |
| Keywords: | Survivin | | radiation | | apoptosis | | dominant negative mutant | | adenoviral vector |
| Issue Date: | 18-Jan-2008 |
| Publisher: | Elsevier |
| Journal Title: | Cancer Letters |
| Volume: | 259 |
| Issue: | 1 |
| Start Page: | 71 |
| End Page: | 81 |
| Publisher DOI: | 10.1016/j.canlet.2007.09.017 |
| PMID: | 17967504 |
| Abstract: | To investigate the mechanism of radioresistance of solid tumor cells, we created two expression vectors encoding Survivin mutants, T34A and D53A. When T34A and D53A were overexpressed in NIH3T3, A549 and HeLa cells, radiation-induced apoptosis was significantly enhanced. Furthermore, we examined the binding capability of Survivin with Smac/DIABLO in the cells that overexpressed these mutants. Coimmunoprecipitation analysis revealed that mutant form of Survivin, D53A and T34A could bind to Smac/DIABLO, but with much less affinity compared to the authentic form. These results suggest that radiation-induced apoptosis of tumor cells is increased by inhibition of the interaction between Survivin and Smac/DIABLO through overexpression of T34A and D53A. |
| Relation: | http://www.sciencedirect.com/science/journal/03043835 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/32876 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 稲波 修
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