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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Enhancement of Cell Death by TNF α-related Apoptosis-inducing Ligand (TRAIL) in Human Lung Carcinoma A549 Cells Exposed to X Rays under Hypoxia
| Title: | Enhancement of Cell Death by TNF α-related Apoptosis-inducing Ligand (TRAIL) in Human Lung Carcinoma A549 Cells Exposed to X Rays under Hypoxia |
| Authors: | Takahashi, Momoko Browse this author | | Inanami, Osamu Browse this author →KAKEN DB | | Kubota, Nobuo Browse this author | | Tsujitani, Michihiko Browse this author | | Yasui, Hironobu Browse this author →KAKEN DB | | Ogura, Aki Browse this author | | Kuwabara, Mikinori Browse this author |
| Keywords: | TRAIL | | radiation | | caspase | | hypoxia |
| Issue Date: | Nov-2007 |
| Publisher: | 日本放射線影響学会 |
| Journal Title: | Journal of Radiation Research |
| Volume: | 48 |
| Issue: | 6 |
| Start Page: | 461 |
| End Page: | 468 |
| Publisher DOI: | 10.1269/jrr.07028 |
| Abstract: | Our previous study showed that ionizing radiation induced the expression of death receptor DR5 on the cell surface in tumor cell lines and that the death receptor of the TNF alpha-related apoptosis-inducing ligand TRAIL enhanced the apoptotic pathway (Hamasu et al., (2005) Journal of Radiation Research, 46:103-110). The present experiments were performed to examine whether treatment with TRAIL enhanced the cell killing in tumor cells exposed to ionizing radiation under hypoxia, since the presence of radioresistant cells in hypoxic regions of solid tumors is a serious problem in radiation therapy for tumors. When human lung carcinoma A549 cells were irradiated under normoxia and hypoxia, respectively, radiation-induced enhancement of expression of DR5 was observed under both conditions. Incubation in the presence of TRAIL enhanced the caspase-dependent and chymotrypsin-like-protease-dependent apoptotic cell death in A549 cells exposed to X rays. Furthermore, it was shown that treatment with TRAIL enhanced apoptotic cell death and loss of clonogenic ability in A549 cells exposed to X rays not only under normoxia but also under hypoxia, suggesting that combination treatment with TRAIL and X irradiation is effective for hypoxic tumor cells. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/32929 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 稲波 修
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