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Embryonic Stages from Cleavage to Gastrula in the Loach Misgunus anguillicaudatus

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Title: Embryonic Stages from Cleavage to Gastrula in the Loach Misgunus anguillicaudatus
Authors: Fujimoto, Takafumi Browse this author
Kataoka, Takashi Browse this author
Otani, Satoshi Browse this author
Saito, Taiju Browse this author
Aita, Takanori Browse this author
Yamaha, Etsuro Browse this author →KAKEN DB
Arai, Katsutoshi Browse this author →KAKEN DB
Keywords: loach
developmental stages
mid-blastula transition (MBT)
no tail
Issue Date: Jul-2004
Publisher: 日本動物学会
Journal Title: Zoological Science
Volume: 21
Issue: 7
Start Page: 747
End Page: 755
Abstract: Early developmental staging from the zygote stage to the gastrula is a basic step for studying embryonic development and biotechnology. We described the early embryonic development of the loach, Misgurnus anguillicaudatus, based on morphological features and gene expression. Synchronous cleavage was repeated for 9 cycles about every 27 min at 20℃ after the first cleavage. After the 10th synchronous cleavage, asynchronous cleavage was observed 5.5 h post-fertilization (hpf), indicating the mid-blastula transition. The yolk syncytial layer (YSL) was formed at this time. Expressions of goosecoid and no tail were detected by whole-mount in situ hybridization from 6 hpf. This time corresponded to the late-blastula period. Thereafter, epiboly started and a blastoderm covered over the yolk cell at 8 hpf. At 10 hpf, the germ ring and the embryonic shield were formed, indicating the stage of early gastrula. Afterward, the epiboly advanced at the rate of 10% of the yolk cell each hour. The blastoderm covered the yolk cell completely at 15 hpf. The embryonic development of the loach resembled that of the zebrafish in terms of morphological change and gene expression. Therefore, it is possible that knowledge of the developmental stages of the zebrafish might be applicable to the loach.
Rights: (c) 日本動物学会 / 本文献の公開は著者の意思に基づくものである
Type: article
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 藤本 貴史

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