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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
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Developmental Stages and Germ Cell Lineage of the Loach (Misgurnus anguillicaudatus)
| Title: | Developmental Stages and Germ Cell Lineage of the Loach (Misgurnus anguillicaudatus) |
| Authors: | Fujimoto, Takafumi Browse this author | | Kataoka, Takashi Browse this author | | Sakao, Suzu Browse this author | | Saito, Taiju Browse this author | | Yamaha, Etsuro Browse this author →KAKEN DB | | Arai, Katsutoshi Browse this author →KAKEN DB |
| Keywords: | loach | | Misgurnus anguillicaudatus | | developmental stage | | germ cell lineage | | PGCs |
| Issue Date: | Nov-2006 |
| Publisher: | 日本動物学会 |
| Journal Title: | Zoological Science |
| Volume: | 23 |
| Issue: | 11 |
| Start Page: | 977 |
| End Page: | 989 |
| Publisher DOI: | 10.2108/zsj.23.977 |
| PMID: | 17189910 |
| Abstract: | The staging of embryonic and larval development, and the germ cell lineage of the loach, Misgurnus anguillicaudatus, are described. Fertilized eggs were obtained by artificial insemination. For the convenience of detailed observation and photography of the external appearance, we use dechorionated embryos. Through a series of operations, these embryos were cultured at 20°C in an incubator. Embryonic and larval development of the loach was divided into five periods: cleavage, blastula, gastrula, segmentation, and hatching. Stages were assigned within each of these periods. Developmental stages were determined and named by morphological features and somite number. The staging series were photographed and tabulated. The germ cell lineage was then elucidated by whole mount in situ hybridization of mRNA expression of the germ-cell-specific marker vasa and histological analysis. Primordial germ cells (PGCs) of the loach derived from the cleavage furrows of 8-cell stage embryos began proliferation in the late blastula period and migrated to the gonadal anlagen through a migration pathway similar to that of the zebrafish. However, it is characteristic of the loach that PGCs migrate a long distance and stay in the posterior part of the yolk-extension region. |
| Rights: | (c) 日本動物学会 / 本文献の公開は著者の意思に基づくものである |
| Relation: | http://www.bioone.org/perlserv/?request=get-archive&issn=0289-0003 |
| Type: | article |
| URI: | http://hdl.handle.net/2115/32972 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 藤本 貴史
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