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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
Peer-reviewed Journal Articles, etc >
Organization of Cytoplasmic Microtubules during Maturation of Goldfish Oocytes
| Title: | Organization of Cytoplasmic Microtubules during Maturation of Goldfish Oocytes |
| Authors: | Jiang, Jian-Qiao Browse this author | | Yamashita, Masakane Browse this author | | Yoshikuni, Michiyasu Browse this author | | Fukada, Sachiko Browse this author | | Nagahama, Yoshitaka Browse this author |
| Issue Date: | Dec-1996 |
| Publisher: | 日本動物学会 |
| Journal Title: | Zoological Science |
| Volume: | 13 |
| Issue: | 6 |
| Start Page: | 899 |
| End Page: | 907 |
| Abstract: | The organization of cytoplasmic microtubules during hormone-induced meiotic maturation of goldfish oocytes in vitro was examined by confocal immunofluorescence microscopy using an anti-tubulin antibody. The microtubule network was well distributed in fully grown immature oocytes. Once goldfish oocytes resumed meiotic maturation by a proposed maturation-inducing hormone of this species (17α, 20β-dihydroxy-4-pregnen-3-one, 17α, 20β-DP), cytoplasmic microtubules serially re-organized. Soon after the onset of the germinal vesicle (GV) migration towards the animal pole, the former microtubule network disappeared, followed by the appearance of a long perinuclear tail with high ordered microtubules extending from the vegetal surface of the GV. Incubation of fully grown immature follilces in colcemid, an inhibitor of tubulin polymerization, caused the disappearance of microtubules. However, this treatment did not prevent either the 17α, 20β-DP-induced migration of the GV or GVBD. Coincident with the breakdown of the GV (GVBD), numerous microtubules intruded into the GV from its vegetal surface. Soon after GVBD, a disk-shaped ring consisting of microtubule asters and a small ring with a radial array of microtubules in its center was observed at the animal pole region. In mature oocytes with meiotic spindles at the animal pole surface, cytoplasmic microtubules were concentrated in a small region around the animal pole showing complicated microtubule arrays. The results presented define distinct changes in microtubule organization during the 17α, 20β-DP-induced meiotic maturation of goldfish oocytes. |
| Rights: | (c) 日本動物学会 / 本文献の公開は著者の意思に基づくものである |
| Relation: | http://www.bioone.org/perlserv/?request=get-archive&issn=0289-0003 |
| Type: | article |
| URI: | http://hdl.handle.net/2115/32998 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 山下 正兼
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