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Organization of Cytoplasmic Microtubules during Maturation of Goldfish Oocytes

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Title: Organization of Cytoplasmic Microtubules during Maturation of Goldfish Oocytes
Authors: Jiang, Jian-Qiao Browse this author
Yamashita, Masakane Browse this author
Yoshikuni, Michiyasu Browse this author
Fukada, Sachiko Browse this author
Nagahama, Yoshitaka Browse this author
Issue Date: Dec-1996
Publisher: 日本動物学会
Journal Title: Zoological Science
Volume: 13
Issue: 6
Start Page: 899
End Page: 907
Abstract: The organization of cytoplasmic microtubules during hormone-induced meiotic maturation of goldfish oocytes in vitro was examined by confocal immunofluorescence microscopy using an anti-tubulin antibody. The microtubule network was well distributed in fully grown immature oocytes. Once goldfish oocytes resumed meiotic maturation by a proposed maturation-inducing hormone of this species (17α, 20β-dihydroxy-4-pregnen-3-one, 17α, 20β-DP), cytoplasmic microtubules serially re-organized. Soon after the onset of the germinal vesicle (GV) migration towards the animal pole, the former microtubule network disappeared, followed by the appearance of a long perinuclear tail with high ordered microtubules extending from the vegetal surface of the GV. Incubation of fully grown immature follilces in colcemid, an inhibitor of tubulin polymerization, caused the disappearance of microtubules. However, this treatment did not prevent either the 17α, 20β-DP-induced migration of the GV or GVBD. Coincident with the breakdown of the GV (GVBD), numerous microtubules intruded into the GV from its vegetal surface. Soon after GVBD, a disk-shaped ring consisting of microtubule asters and a small ring with a radial array of microtubules in its center was observed at the animal pole region. In mature oocytes with meiotic spindles at the animal pole surface, cytoplasmic microtubules were concentrated in a small region around the animal pole showing complicated microtubule arrays. The results presented define distinct changes in microtubule organization during the 17α, 20β-DP-induced meiotic maturation of goldfish oocytes.
Rights: (c) 日本動物学会 / 本文献の公開は著者の意思に基づくものである
Type: article
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山下 正兼

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