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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
Peer-reviewed Journal Articles, etc >
Involvement of 26S Proteasome in Oocyte Maturation of Goldfish Carassius auratus
| Title: | Involvement of 26S Proteasome in Oocyte Maturation of Goldfish Carassius auratus |
| Authors: | Tokumoto, Mika Browse this author | | Horiguchi, Ryo Browse this author | | Yamashita, Masakane Browse this author | | Nagahama, Yoshitaka Browse this author | | Tokumoto, Toshinobu Browse this author |
| Issue Date: | Apr-1997 |
| Publisher: | 日本動物学会 |
| Journal Title: | Zoological Science |
| Volume: | 14 |
| Issue: | 2 |
| Start Page: | 347 |
| End Page: | 351 |
| Abstract: | Diisopropyl fluorophosphate (DFP), a serine protease inhibitor, inhibited the activity of 26S proteasome in goldfish oocytes and arrested the oocytes at two distinct stages of maturation. One was prior to the migration of germinal vesicle (GV) toward the animal pole, and the other was between the attachment of GV to the oocyte plasma membrane and GV breakdown (GVBD). The first DFP-sensitive period corresponded to the period during which the activity of proteasomes increased, but maturation-promoting factor (MPF) was still inactive. During the second DFP-sensitive period, MPF was abruptly activated, although the proteasome activity detectable in the oocyte cytosol extracted by ultracentrifugation reached the lowest level during this period. These results suggest the requirement of 26S proteasome for at least two stages of oocyte maturation, the early stage before GV migration and the later stage, including MPF activation immediately before GVBD. |
| Rights: | (c) 日本動物学会 / 本文献の公開は著者の意思に基づくものである |
| Relation: | http://www.bioone.org/perlserv/?request=get-archive&issn=0289-0003 |
| Type: | article |
| URI: | http://hdl.handle.net/2115/32999 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 山下 正兼
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