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Involvement of 26S Proteasome in Oocyte Maturation of Goldfish Carassius auratus

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Title: Involvement of 26S Proteasome in Oocyte Maturation of Goldfish Carassius auratus
Authors: Tokumoto, Mika Browse this author
Horiguchi, Ryo Browse this author
Yamashita, Masakane Browse this author
Nagahama, Yoshitaka Browse this author
Tokumoto, Toshinobu Browse this author
Issue Date: Apr-1997
Publisher: 日本動物学会
Journal Title: Zoological Science
Volume: 14
Issue: 2
Start Page: 347
End Page: 351
Abstract: Diisopropyl fluorophosphate (DFP), a serine protease inhibitor, inhibited the activity of 26S proteasome in goldfish oocytes and arrested the oocytes at two distinct stages of maturation. One was prior to the migration of germinal vesicle (GV) toward the animal pole, and the other was between the attachment of GV to the oocyte plasma membrane and GV breakdown (GVBD). The first DFP-sensitive period corresponded to the period during which the activity of proteasomes increased, but maturation-promoting factor (MPF) was still inactive. During the second DFP-sensitive period, MPF was abruptly activated, although the proteasome activity detectable in the oocyte cytosol extracted by ultracentrifugation reached the lowest level during this period. These results suggest the requirement of 26S proteasome for at least two stages of oocyte maturation, the early stage before GV migration and the later stage, including MPF activation immediately before GVBD.
Rights: (c) 日本動物学会 / 本文献の公開は著者の意思に基づくものである
Type: article
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山下 正兼

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