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Point mutations in helper component protease of clover yellow vein virus are associated with the attenuation of RNA-silencing suppression activity and symptom expression in broad bean
Title: | Point mutations in helper component protease of clover yellow vein virus are associated with the attenuation of RNA-silencing suppression activity and symptom expression in broad bean |
Authors: | Yambao, M. L. M. Browse this author | Yagihashi, H. Browse this author | Sekiguchi, H. Browse this author | Sekiguchi, T. Browse this author | Sasaki, T. Browse this author | Sato, M. Browse this author | Atsumi, G. Browse this author | Tacahashi, Y. Browse this author | Nakahara, K. S. Browse this author →KAKEN DB | Uyeda, I. Browse this author →KAKEN DB |
Issue Date: | Jan-2008 |
Publisher: | Springer Wien |
Journal Title: | Archives of Virology |
Volume: | 153 |
Issue: | 1 |
Start Page: | 105 |
End Page: | 115 |
Publisher DOI: | 10.1007/s00705-007-1073-3 |
PMID: | 17955160 |
Abstract: | Helper component protease (HC-Pro) is a potyvirus-encoded multifunctional protein and a major determinant of symptom expression in a susceptible plant. Here, we show the involvement of clover yellow vein virus (ClYVV) HC-Pro in necrotic symptom expression in broad bean (Vicia faba cv. Wase). In this host, lethal necrosis was induced by ClYVV no. 30, from which a spontaneous, mosaic-inducing mutant (MM) was obtained. Mapping with chimeric viruses between ClYVV no. 30 and MM attributed the symptom attenuation to two mutations at the HC-Pro positions 27 (threonine to isoleucine) and 193 (aspartic acid to tyrosine). Although neither mutant with the single amino acid substitution at position 27 or 193 (ClYVV/T27I or D193Y) induced the lethal necrosis, ClYVV/T27I still retained the ability to induce necrotic symptoms, but ClYVV/D193Y scarcely did so. The virus accumulation of ClYVV/D193Y was also lower than that of ClYVV no. 30. The mutations, T27I and D193Y, are located in a putative zinc finger domain and in one (N-terminal) of the two RNA binding domains, respectively, of HC-Pro. RNA-silencing suppression (RSS) activity of P1/HC-Pro in Nicotiana benthamiana was weakened by both mutations. Our results suggest a correlation between viral virulence and RSS function and the importance of the two domains in HC-Pro. |
Rights: | The original publication is available at www.springerlink.com |
Relation: | http://www.springerlink.com |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/33039 |
Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 中原 健二
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