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Volume 56, Number 1 >

Availability of oral swab sample for the detection of bovine viral diarrhea virus (BVDV) gene from the cattle persistently infected with BVDV

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.56.1.3

Title: Availability of oral swab sample for the detection of bovine viral diarrhea virus (BVDV) gene from the cattle persistently infected with BVDV
Authors: Tajima, Motoshi Browse this author →KAKEN DB
Ohsaki, Tomohiro Browse this author
Okazawa, Manabu Browse this author
Yasutomi, Ichiro Browse this author
Keywords: bovine viral diarrhea virus
BVDV
gene detection
swab sampling
Issue Date: May-2008
Publisher: The Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 56
Issue: 1
Start Page: 3
End Page: 8
Abstract: Bovine nasal and oral discharges were used as samples for bovine viral diarrhea virus (BVDV) gene detection. Viral genes in serum (S), nasal discharge (N) and oral discharge (O) were quantified with real-time polymerase chain reaction using SYBR Green by the relative quantification method, and findings were compared among samples. Although the quantity of the BVDV gene in S was greater than those in N and O, all samples were available to identify persistently infected (PI) cattle with BVDV by reverse transcription polymerase chain reaction (RT-PCR). The swab samples were able to be stored for a few days at 4℃with a little decrease of amplification signal in RT-PCR. Oral swab sampling was easier than nasal swab sampling, and was also less uncomfortable for the cattle than other sampling methods without pain or unnecessary retention. This sampling method can be performed without any special technique and equipment. Therefore, the oral swab sampling method is useful for screening to detect BVDV PI cattle by RT-PCR.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/33848
Appears in Collections:Japanese Journal of Veterinary Research > Volume 56, Number 1

Submitter: 獣医学部図書室

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