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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
A deletion mutation of the protein tyrosine phosphatase kappa (Ptprk) gene is responsible for the T-helper immunodeficiency (thid) in the LEC rat
| Title: | A deletion mutation of the protein tyrosine phosphatase kappa (Ptprk) gene is responsible for the T-helper immunodeficiency (thid) in the LEC rat |
| Authors: | Asano, Atsushi Browse this author | | Tsubomatsu, Kouta Browse this author | | Jung, Cha-Gyun Browse this author | | Sasaki, Nobuya Browse this author →KAKEN DB | | Agui, Takashi Browse this author →KAKEN DB |
| Issue Date: | Nov-2007 |
| Publisher: | Springer New York |
| Journal Title: | Mammalian Genome |
| Volume: | 18 |
| Issue: | 11 |
| Start Page: | 779 |
| End Page: | 786 |
| Publisher DOI: | 10.1007/s00335-007-9062-0 |
| PMID: | 17909891 |
| Abstract: | Bone marrow (BM)-derived T-cell progenitors differentiate into CD4 or CD8 single positive (SP) cells in the thymus. We have previously reported that a single autosomal mutation, thid, causes a defect in maturation of CD4 SP thymocytes and an abnormality of peripheral helper-T cells in the LEC rat. In this study, we attempted to identify a gene responsible for the thid mutation. We first performed genetic linkage analysis and mapped thid locus between Myb and D1Rat392 on Chr 1. In this region, we found a ~380 kb deletion from intron 3 of the Ptprk gene, which encodes a receptor-like protein tyrosine phosphatase type κ (RPTPκ), to intron 1 of RGD1560849 predicted gene in the LEC rat genome. Reconstitution with syngenic BM cells transduced Ptprk but not RGD1560849 predicted gene rescued development of CD4 SP cells in the LEC rat thymus. It is confirmed by this result that the Ptprk gene is responsible for the thid mutation in the LEC rat. Our results further suggest that RPTPκ plays a critical role in the development of CD4 SP cells in the thymus. |
| Rights: | The original publication is available at www.springerlink.com |
| Relation: | http://www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/33866 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 安居院 高志
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