HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >

A deletion mutation of the protein tyrosine phosphatase kappa (Ptprk) gene is responsible for the T-helper immunodeficiency (thid) in the LEC rat

Files in This Item:
thidMS(revised).pdf1.25 MBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/33866

Title: A deletion mutation of the protein tyrosine phosphatase kappa (Ptprk) gene is responsible for the T-helper immunodeficiency (thid) in the LEC rat
Authors: Asano, Atsushi Browse this author
Tsubomatsu, Kouta Browse this author
Jung, Cha-Gyun Browse this author
Sasaki, Nobuya Browse this author →KAKEN DB
Agui, Takashi Browse this author →KAKEN DB
Issue Date: Nov-2007
Publisher: Springer New York
Journal Title: Mammalian Genome
Volume: 18
Issue: 11
Start Page: 779
End Page: 786
Publisher DOI: 10.1007/s00335-007-9062-0
PMID: 17909891
Abstract: Bone marrow (BM)-derived T-cell progenitors differentiate into CD4 or CD8 single positive (SP) cells in the thymus. We have previously reported that a single autosomal mutation, thid, causes a defect in maturation of CD4 SP thymocytes and an abnormality of peripheral helper-T cells in the LEC rat. In this study, we attempted to identify a gene responsible for the thid mutation. We first performed genetic linkage analysis and mapped thid locus between Myb and D1Rat392 on Chr 1. In this region, we found a ~380 kb deletion from intron 3 of the Ptprk gene, which encodes a receptor-like protein tyrosine phosphatase type κ (RPTPκ), to intron 1 of RGD1560849 predicted gene in the LEC rat genome. Reconstitution with syngenic BM cells transduced Ptprk but not RGD1560849 predicted gene rescued development of CD4 SP cells in the LEC rat thymus. It is confirmed by this result that the Ptprk gene is responsible for the thid mutation in the LEC rat. Our results further suggest that RPTPκ plays a critical role in the development of CD4 SP cells in the thymus.
Rights: The original publication is available at www.springerlink.com
Relation: http://www.springerlink.com
Type: article (author version)
URI: http://hdl.handle.net/2115/33866
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 安居院 高志

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

 - Hokkaido University