|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Environmental Science / Faculty of Environmental Earth Science >
Peer-reviewed Journal Articles, etc >
An EntD-like phosphopantetheinyl transferase gene from Photobacterium profundum SS9 complements pfa genes of Moritella marina strain MP-1 involved in biosynthesis of docosahexaenoic acid
| Title: | An EntD-like phosphopantetheinyl transferase gene from Photobacterium profundum SS9 complements pfa genes of Moritella marina strain MP-1 involved in biosynthesis of docosahexaenoic acid |
| Authors: | Sugihara, Shinji Browse this author | | Orikasa, Yoshitake Browse this author | | Okuyama, Hidetoshi Browse this author →KAKEN DB |
| Keywords: | EntD | | pfaE | | phosphopantetheinyl transferase | | Photobacterium profundum SS9 | | polyunsaturated fatty acid |
| Issue Date: | Mar-2008 |
| Publisher: | Springer Netherlands |
| Journal Title: | Biotechnology Letters |
| Volume: | 30 |
| Issue: | 3 |
| Start Page: | 411 |
| End Page: | 414 |
| Publisher DOI: | 10.1007/s10529-007-9579-z |
| PMID: | 17973084 |
| Abstract: | The EntD-like phosphopantetheinyl transferase (PPTase) gene, cloned from the eicosapentaenoic acid-producing bacterium Photobacterium profundum strain SS9, has an open reading frame of 690 bp encoding a 230-amino acid protein. When this PPTase gene was expressed in Escherichia coli with pfaA, pfaB, pfaC and pfaD derived from Moritella marina MP-1, which were four of five essential genes for biosynthesis of docosahexaenoic acid (DHA), the DHA production of the recombinant was 2.0% (w/w) of total fatty acids. This is the first report showing that the EntD-like PPTase is involved in producing n-3 polyunsaturated fatty acids. |
| Rights: | The original publication is available at www.springerlink.com |
| Relation: | http://www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/33869 |
| Appears in Collections: | 環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 奥山 英登志
|
