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An EntD-like phosphopantetheinyl transferase gene from Photobacterium profundum SS9 complements pfa genes of Moritella marina strain MP-1 involved in biosynthesis of docosahexaenoic acid

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Title: An EntD-like phosphopantetheinyl transferase gene from Photobacterium profundum SS9 complements pfa genes of Moritella marina strain MP-1 involved in biosynthesis of docosahexaenoic acid
Authors: Sugihara, Shinji Browse this author
Orikasa, Yoshitake Browse this author
Okuyama, Hidetoshi Browse this author →KAKEN DB
Keywords: EntD
pfaE
phosphopantetheinyl transferase
Photobacterium profundum SS9
polyunsaturated fatty acid
Issue Date: Mar-2008
Publisher: Springer Netherlands
Journal Title: Biotechnology Letters
Volume: 30
Issue: 3
Start Page: 411
End Page: 414
Publisher DOI: 10.1007/s10529-007-9579-z
PMID: 17973084
Abstract: The EntD-like phosphopantetheinyl transferase (PPTase) gene, cloned from the eicosapentaenoic acid-producing bacterium Photobacterium profundum strain SS9, has an open reading frame of 690 bp encoding a 230-amino acid protein. When this PPTase gene was expressed in Escherichia coli with pfaA, pfaB, pfaC and pfaD derived from Moritella marina MP-1, which were four of five essential genes for biosynthesis of docosahexaenoic acid (DHA), the DHA production of the recombinant was 2.0% (w/w) of total fatty acids. This is the first report showing that the EntD-like PPTase is involved in producing n-3 polyunsaturated fatty acids.
Rights: The original publication is available at www.springerlink.com
Relation: http://www.springerlink.com
Type: article (author version)
URI: http://hdl.handle.net/2115/33869
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 奥山 英登志

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