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Hokkaido University Collection of Scholarly and Academic Papers >
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A turbine oil-degrading bacterial consortium from soils of oil fields and its characteristics
| Title: | A turbine oil-degrading bacterial consortium from soils of oil fields and its characteristics |
| Authors: | Ito, Hitoshi Browse this author | | Hosokawa, Reia Browse this author | | Morikawa, Masaaki Browse this author →KAKEN DB | | Okuyama, Hidetoshi Browse this author →KAKEN DB |
| Keywords: | Bacterial consortia | | Oil field | | Turbine oil (TuO) | | Turbine Oil-degrading bacteria | | Unculturable bacteria |
| Issue Date: | Apr-2008 |
| Publisher: | Elsevier |
| Journal Title: | International Biodeterioration & Biodegradation |
| Volume: | 61 |
| Issue: | 3 |
| Start Page: | 223 |
| End Page: | 232 |
| Publisher DOI: | 10.1016/j.ibiod.2007.08.001 |
| Abstract: | A microbial consortium capable of degrading turbine oil (TuO), which consisted mainly of recalcitrant cycloalkanes and isoalkanes, was obtained from a soil sample collected from oil fields using repeated enrichment. When this consortium, named Atsuta A, was cultured in mineral salts medium containing 0.5% (w/v) TuO, it degraded 90% of TuO at 30℃ and pH 7 over 5 days. Although nine bacterial strains were isolated from the Atsuta A consortium, TuO degradation by the individual isolates and by a mixture of them was negligible. The community structure of the consortium, which was investigated by PCR–denaturing gradient gel electrophoresis (DGGE) targeting 16S rRNA genes, changed significantly during the degradation of TuO. Four major bands (F, K, N and T) out of at least 23 visible DGGE bands significantly increased in intensity over time during incubation. The DGGE bands F, K and N corresponded to those of previously isolated species. However, DGGE band T did not correspond to any isolated strain. The 16S rRNA gene sequence collected from band T was 98% homologous to that of an unculturable strain belonging to the γ-Proteobacteria. The degradation of TuO in the consortium may occur by cooperation between the unculturable species corresponding to band T and other strains in the consortium, including species corresponding to bands F, K and N. |
| Relation: | http://www.sciencedirect.com/science/journal/09648305 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/33870 |
| Appears in Collections: | 環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 奥山 英登志
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