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A turbine oil-degrading bacterial consortium from soils of oil fields and its characteristics

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Title: A turbine oil-degrading bacterial consortium from soils of oil fields and its characteristics
Authors: Ito, Hitoshi Browse this author
Hosokawa, Reia Browse this author
Morikawa, Masaaki Browse this author →KAKEN DB
Okuyama, Hidetoshi Browse this author →KAKEN DB
Keywords: Bacterial consortia
Oil field
Turbine oil (TuO)
Turbine Oil-degrading bacteria
Unculturable bacteria
Issue Date: Apr-2008
Publisher: Elsevier
Journal Title: International Biodeterioration & Biodegradation
Volume: 61
Issue: 3
Start Page: 223
End Page: 232
Publisher DOI: 10.1016/j.ibiod.2007.08.001
Abstract: A microbial consortium capable of degrading turbine oil (TuO), which consisted mainly of recalcitrant cycloalkanes and isoalkanes, was obtained from a soil sample collected from oil fields using repeated enrichment. When this consortium, named Atsuta A, was cultured in mineral salts medium containing 0.5% (w/v) TuO, it degraded 90% of TuO at 30℃ and pH 7 over 5 days. Although nine bacterial strains were isolated from the Atsuta A consortium, TuO degradation by the individual isolates and by a mixture of them was negligible. The community structure of the consortium, which was investigated by PCR–denaturing gradient gel electrophoresis (DGGE) targeting 16S rRNA genes, changed significantly during the degradation of TuO. Four major bands (F, K, N and T) out of at least 23 visible DGGE bands significantly increased in intensity over time during incubation. The DGGE bands F, K and N corresponded to those of previously isolated species. However, DGGE band T did not correspond to any isolated strain. The 16S rRNA gene sequence collected from band T was 98% homologous to that of an unculturable strain belonging to the γ-Proteobacteria. The degradation of TuO in the consortium may occur by cooperation between the unculturable species corresponding to band T and other strains in the consortium, including species corresponding to bands F, K and N.
Relation: http://www.sciencedirect.com/science/journal/09648305
Type: article (author version)
URI: http://hdl.handle.net/2115/33870
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 奥山 英登志

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