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Functional analysis of N-terminal domains of Arabidopsis chlorophyllide a oxygenase
Title: | Functional analysis of N-terminal domains of Arabidopsis chlorophyllide a oxygenase |
Authors: | Sakuraba, Yasuhito Browse this author | Yamasato, Akihiro Browse this author | Tanaka, Ryouichi Browse this author →KAKEN DB | Tanaka, Ayumi Browse this author →KAKEN DB |
Keywords: | Chlorophyllide a oxygenase | Chlorophyll cycle | Arabidopsis |
Issue Date: | Oct-2007 |
Publisher: | Elsevier |
Journal Title: | Plant Physiology and Biochemistry |
Volume: | 45 |
Issue: | 10-11 |
Start Page: | 740 |
End Page: | 749 |
Publisher DOI: | 10.1016/j.plaphy.2007.07.016 |
PMID: | 17884554 |
Abstract: | Higher plants acclimate to various light environments by changing the antenna size of a light harvesting photosystem. The antenna size of a photosystem is partly determined by the amount of chlorophyll b in the light-harvesting complexes. Chlorophyllide a oxygenase (CAO) converts chlorophyll a to chlorophyll b in a two-step oxygenation reaction. In our previous study, we demonstrated that the cellular level of the CAO protein controls accumulation of chlorophyll b. We found that the amino acids sequences of CAO in higher plants consist of three domains (A, B, and C domains). The C domain exhibits a catalytic function, and we demonstrated that the combination of the A and B domains regulates the cellular level of CAO. However, the individual function of each of A and B domain has not been determined yet. Therefore, in the present study we constructed a series of deleted CAO sequences that were fused with green fluorescent protein and overexpressed in a chlorophyll b-less mutant of Arabidopsis thaliana, ch1-1, to further dissect functions of A and B domains. Subsequent comparative analyses of the transgenic plants overexpressing B-domain containing proteins and those lacking the B domain determined that there was no significant difference in CAO protein levels. These results indicate that the B domain is not involved in the regulation of the CAO protein levels. Taken together, we concluded that the A domain alone is involved in the regulatory mechanism of the CAO protein levels. |
Relation: | http://www.sciencedirect.com/science/journal/09819428 |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/33905 |
Appears in Collections: | 低温科学研究所 (Institute of Low Temperature Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 田中 亮一
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