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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
X Irradiation Combined with TNF α-related Apoptosis-inducing Ligand (TRAIL) Reduces Hypoxic Regions of Human Gastric Adenocarcinoma Xenografts in SCID Mice
| Title: | X Irradiation Combined with TNF α-related Apoptosis-inducing Ligand (TRAIL) Reduces Hypoxic Regions of Human Gastric Adenocarcinoma Xenografts in SCID Mice |
| Authors: | TAKAHASHI, Momoko Browse this author | | YASUI, Hironobu Browse this author | | OGURA, Aki Browse this author | | ASANUMA, Taketoshi Browse this author | | KUBOTA, Nobuo Browse this author | | TSUJITANI, Michihiko Browse this author | | KUWABARA, Mikinori Browse this author | | INANAMI, Osamu Browse this author →KAKEN DB |
| Keywords: | Radiation | | Solid tumor | | TRAIL | | Hypoxia | | Xenograft |
| Issue Date: | 26-Mar-2008 |
| Publisher: | Japan Radiation Research Society |
| Journal Title: | Journal of Radiation Research |
| Volume: | 49 |
| Issue: | 2 |
| Start Page: | 153 |
| End Page: | 161 |
| Publisher DOI: | 10.1269/jrr.07082 |
| Abstract: | Our previous study showed that X irradiation induced the expression of death receptor DR5 on the cell surface in tumor cell lines under not only normoxia but also hypoxia. X irradiation combined with TNF α-related apoptosis-inducing ligand (TRAIL), which is the ligand of DR5, induced apoptosis in vitro (Takahashi et al., (2007) Journal of Radiation Research, 48: 461-468). In this report, we examined the in vivo antitumor efficacy of X irradiation combined with TRAIL treatment in tumor xenograft models derived from human gastric adenocarcinoma MKN45 and MKN28 cells in SCID mice. X irradiation combined with TRAIL synergistically suppressed the tumor growth rates in the xenograft models derived from MKN45 and MKN28 cells, which have wild type Tp53 and mutated Tp53, respectively, indicating that the antitumor effects occurred in a Tp53-independent manner. Histological analysis showed that the combination of X irradiation and TRAIL induced caspase-3-dependent apoptotic cell death. Moreover, the immunohistochemical detection of hypoxic regions using the hypoxic marker pimonidazole revealed that caspase-3-dependent apoptosis occurred in the hypoxic regions in the tumors. These results indicated that X irradiation combined with TRAIL may be a useful treatment to reduce tumor growth in not only normoxic but also hypoxic regions. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/33951 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 稲波 修
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